Cloning your gene can be one of the most difficult parts of an experiment. The first step is to decide which method will be most effective for inserting your gene precisely at the target locus. 

New to molecular cloning? View our comprehensive guide that includes the fundamentals of molecular cloning, including restriction digestion, DNA ligation, vector dephosphorylation, and bacterial transformation.

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Restriction Enzyme Cloning

Restriction enzymes (or restriction endonucleases) recognize specific sequences of DNA and cleave the strands at sites within or adjacent to those sequences. When you're using restriction enzymes, your reaction conditions will need to be adjusted to the needs of the specific enzyme you're working with. 

Molecular Biology Lab Guide

From basic DNA purification to the most complicated qPCR experiment, the Molecular Biology Lab Guide can help you make every experiment successful.

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