Cloning your gene can be one of the most difficult parts of an experiment. The first step is to decide which method will be most effective for inserting your gene precisely at the target locus.
PCR cloning is a fast and efficient method that doesn't require restriction enzymes. These blogs tackle some of the biggest challenges, like designing the right insert and optimizing the reaction conditions.
PCR Cloning FAQ
Find the answers to (almost) everything you’ve ever wondered about PCR cloning.
PCR Cloning Guide
Jump into the PCR Cloning section of our guide to subcloning and learn about the techniques and products you’ll need to be successful.
A Quick Method for A-Tailing PCR Products
Explore a few strategies you might not have considered to overcome the challenges of “A” overhangs.
Learn the basics of T-Vector Cloning in these blog responses to frequently asked questions.
Cloning with pGEM-T Vectors: Ligation
Learn how to ligate your blunt-ended DNA fragments using pGEM-T vectors.
T-Vector Cloning FAQ
Need more tips for successful cloning? Check out these answers to frequently asked questions.
Cloning Modified Blunt-Ended DNA Fragments into T-Vectors
Read about a method for eliminating some of the requirements of conventional blunt-end cloning.
Restriction Enzyme Cloning
Restriction enzymes recognize specific sequences of DNA and cleave the strands at sites within or adjacent to those sequences. When you're using restriction enzymes, your reaction conditions will need to be adjusted to the needs of the specific enzyme you're working with.
Tips for Restriction Enzyme-Digested Vectors and Inserts
Read a few useful tips from some restriction enzyme experts.
How to Set-Up a Restriction Enzyme Digest
Visit our YouTube channel for a quick guide to working with restriction enzymes.
Restriction Enzyme Digestion Applications and Resources
Explore applications for restriction enzyme digests and more in this resource to help you conquer your research.
Molecular Biology Lab Guide
From basic DNA purification to the most complicated qPCR experiment, the Molecular Biology Lab Guide can help you make every experiment successful.
Ready to start? Check out these tools, which can help you prepare for every step of cloning.