Nucleic Acid Purification and Quantitation

In today’s world of nucleic acid analysis by real-time PCR and next-generation sequencing, it’s crucial to purify high-quality nucleic acids from your samples. Once you accomplish that, virtually every downstream assay requires that you quantitate your DNA or RNA before you begin. Understanding how these systems work and then choosing the best chemistry for your application is essential.


RNA Purification

RNA is found in the nucleus, cytoplasm and mitochondria of eukaryotic cells. For use in downstream assays, RNA must be free of DNA and potential inhibitors that can interfere with labeling or hybridization.

A piece of single-stranded RNA floats across the screen


The "Hows" and "Whys" of RNA Analysis

Hear experts discuss the early steps of purification, protection from degradation and quantitation to improve your downstream analysis.

A piece of single-stranded RNA floats across the screen and then pauses


How to Isolate RNA Like a Pro

Improve your RNA isolation with tips from someone who's been there.

A piece of single-stranded RNA floats from the top of the screen, down


Working with RNA

RNase troubles? Check out a few tips for easier RNA analysis.

Molecular Biology Lab Guide

From basic DNA purification to the most complicated qPCR experiment, the Molecular Biology Lab Guide can help you make every experiment successful.

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Nucleic Acid Quantitation

Molecular Biology Rule #1: Never skip quantitating your nucleic acids. This important step helps you make sure you're ready for your next application. Maybe you'll get lucky, but you risk having to start over when you skip quantitation. No one wants to do that.

Quantitation Blogs

Did we mention that quantitation is important? That's why our bloggers keep writing posts about quantitation for different applications.