Formalin-fixed paraffin-embedded (FFPE) tissue samples are a valuable source of genetic information for gene expression and clinical research. Extraction of nucleic acid from FFPE tissues is a challenge because the fixation process results in cross-linking between proteins and nucleic acid, as well as between different strands of DNA or RNA molecules. Damage to the nucleic acid also results in a high degree of fragmentation making it difficult to extract nucleic acid that is suitable for downstream applications such as genotyping, gene expression profiling and Next Generation Sequencing.
Recent advances in sample preparation now enable access to the valuable information contained within these difficult samples. In our webinar we will discuss FFPE samples, the challenges that need to be addressed for success, and approaches to consider for optimizing your research, including:
- Fixation and embedding
- Nucleic acid extraction
- Weighing the pros and cons of different quantitation systems
- Analysis of FFPE samples by qPCR
- Suitability for other demanding downstream assays such as sequencing
Trista Schagat, PhD
Scientific Applications Manager
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