To Nanodrop® or Not to Nanodrop®: Choosing the Most Appropriate Method for Nucleic Acid Quantitation

In this webinar, you will learn:

  • Advantages and disadvantages of different sample quantitation methods
  • How to choose the right sample quantitation method(s) for different samples
  • How to interpret and apply sample quantitation information for downstream analysis success


Accurate and reliable nucleic acid quantitation is a crucial step in DNA and RNA analysis workflows.  Often success or failure in downstream applications simply comes down to whether or not the appropriate amount of input nucleic acid is used.  There are a number of key considerations when quantifying nucleic acids with the goal being to obtain not only an accurate measurement of yield/concentration, but often integrity and purity as well. Not all quantitation methods reveal the same information about a sample, so understanding the strengths and weaknesses of each method allows a researcher to choose an appropriate method(s) based on the starting sample type and downstream assay. This webinar will review a variety of quantitation methods including absorbance, fluorescent nucleic acid-binding dyes and qPCR.  The advantages and disadvantages of each method will be discussed including data from samples that were quantitated using multiple methods to highlight the differences in these methods. Knowing how to apply the most informative sample quantitation techniques to different samples is the key to success.



Jennifer Mook
Senior Applications Scientist

Jennifer Mook is a Senior Applications Scientist at Promega. Jennifer has been a member of the Scientific Applications team since 2018. Here, she utilizes Promega’s broad portfolio of products by adapting them for custom workflows to meet customers’ unique needs. Prior to joining Promega, Jennifer received her B.S. in Cellular and Molecular Biology from Winona State University, then worked for a large agricultural company and a medical diagnostic company. In 2009, Jennifer joined Promega as an R&D Scientist, focusing on nucleic acid amplification technologies, launching several products including the MethylEdge® Bisulfite Conversion System and ProNex® DNA QC Assay.

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