Real-time PCR (qPCR) has become the technique of choice for a variety of applications such a gene expression analysis, genotyping, specific target sequence detection/quantitation and overall nucleic acid quantitation. This webinar will cover multiple qPCR topics aimed at improving your qPCR results including: optimizing qPCR assays including whether to use probe- or dye-based detection, choosing between 1-step and 2-step RT-qPCR assays, and overcoming key challenges such as sample integrity, multiplexing and the presence of inhibitors in the input sample. We will also introduce the GoTaq® family of qPCR and RT-qPCR systems which help researchers overcome these challenges.
Sr Research Scientist
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