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Introduction to Real-Time PCR: Basic Principles and Chemistries


Real-time PCR (qPCR) is the workhorse of most gene expression studies.  However, it can be overwhelming to a new user given the requirement for advanced instruments, vast choices for reagents, and the complexity of the technique.

This webinar will provide an overview of basic qPCR principles compared to traditional PCR, instruments, reagents considerations, and common terms used in assay design and analysis.



Leta Steffen, PhD
Applications Scientist
Promega Corporation

Leta is an Applications Scientist at Promega Corporation based in Madison, WI. She received a B.A. in Biology and Physics from Lawrence University and a Ph.D. in Genetics from Harvard University. As a graduate student, Leta mapped novel genetic mutations causing muscular dystrophy, using zebrafish as a model organism. Leta has been with Promega for 8 years, focusing on nucleic acid amplification technologies.

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