This endoproteinase hydrolyzes peptide bonds on the N-terminal side of aspartic and to a lesser extent glutamic acid residues (Asp and Glu). Asp-N activity is optimal in the pH range of 4.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in solution or in gel.
- Peptide mapping
- Protein identification