We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

United States
Get Up to $500 of Free Promega Reagents with our Back to the Bench Program. Register Now ›

Asp-N, Sequencing Grade

Hydrolyzes Peptide Bonds on the N-Terminal Side of Aspartic and Cysteic Acid Residues (Asp and Cys)

  • Optimal activity in pH range of 4–9
  • Sequencing grade enzyme for use alone or in combination with other proteases
  • Use for in-solution or in-gel digestion reactions

Get Up to $500 of Free Promega Reagents

To support the scientific community, we’re offering free lab reagents! Sign up before July 31, 2020 to get up to $500 of free Promega reagents for your lab. *For U.S. and Canada customers only.*

Size

Catalog number selected: V1621

$ 188.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

Already a Helix Customer?

Request to add this product to your Helix »
Asp-N, Sequencing Grade
2μg
$ 188.00
Your price: Log in

This endoproteinase hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues (Asp and Cys). Asp-N activity is optimal in the pH range of 4.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in solution or in gel.

Applications

  • Peptide mapping
  • Protein identification

 

Protocols

Complete Protocol

Specifications

What's in the box?

Item Part # Size

Asp-N, Sequencing Grade

 V162A 1 × 2μg

SDS

Choose language:

Download SDSPDF (49 KB) – English (United States)

Certificate of Analysis

See all certificates for V1621

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

Resources

Other Resources

Similar Products

Endoglycosidase H

Characterize protein glycosylation in applications such as monitoring protein trafficking and determining glycan location and structure by mass spectrometry.

V4871, V4875

PNGase F

Catalyzes cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues.

V4831

rAsp-N, Mass Spec Grade

Cleavage site: N-terminal of Asp. Optimal pH: 6–9.

VA1160

Trypsin Gold, Mass Spectrometry Grade

Maximum digest specificity with extreme resistance to autolytic digestion.

V5280

Trypsin/Lys-C Mix, Mass Spec Grade

Cleavage sites: C-terminal of Arg and Lys. Optimal pH: 8.

V5071, V5072, V5073

ProteaseMAX™ Surfactant, Trypsin Enhancer

For improved in-gel digestion and protein solubilization.

V2071, V2072

Frequently Used With

Rapid Digestion-Trypsin and -Trypsin/Lys-C Kits

Minimize protein digestion time, maximize data output.

VA1060, VA1061

Immobilized Trypsin

Quick, convenient method for digesting a range of purified protein or complex protein mixture concentrations.

V9012, V9013

Glu-C, Sequencing Grade

Cleavage site: C-terminal of Glu. Optimal pH: 4–9.

V1651

rLys-C, Mass Spec Grade

Cleavage site: C-terminal of Lys. Optimal pH: 8–9.

V1671

Sequencing Grade Modified Trypsin

Cleavage sites: C-terminal of Arg and Lys. Optimal pH: 7–9.

V5111, V5117, V5113

Let's find the product that meets your needs.

Talk to a Scientist

Joliene

Joliene

US
Choose your country

Americas

Brazil
/
Canada
/
United States

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
Estonia
Finland
France
/
Germany
/
Iceland
Italy
/
Luxembourg
Netherlands
Norway
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom