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PNGase F

V4831_PNGase-F_3
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Recombinant Glycosidase Cloned from Elizabethkingia miricola

  • Use to determine protein glycosylation status and location
  • Cleaves N-linked oligosaccharides between innermost GlcNAc and asparagines from N-linked glycoproteins
  • Will not remove oligosaccharides found on plant glycoproteins
  • Supplied at a concentration of 10u/μl

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Catalog number selected: V4831

$ 171.00
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PNGase F
500 units
$ 171.00
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PNGase F, Recombinant Glycosidase

PNGase F is a recombinant glycosidase cloned from Elizabethkingia miricola and overexpressed in E. coli. PNGase F has a molecular weight of 36kDa. PNGase F catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins (see figure). PNGase F will not remove oligosaccharides containing Alpha-(1,3)-linked core fucose commonly found on plant glycoproteins.

Unit Definition: One unit of PNGase F will catalyze the deglycosylation of 1 nanomole of denatured Ribonuclease B (RNase B) in one minute at 37°C. One Promega unit is equal to 1 IUB milliunit.

Molecular Weight: PNGase F has a molecular weight of approximately 36kDa.

Physical Form: PNGase F is supplied as a liquid in 20mM Tris-HCl (pH 7.5 at 25°C), 50mM NaCl and 5mM EDTA at a concentration of 10,000u/ml.

Applications
  • Characterizing whether the protein is glycosylated
  • Determining the location of glycosylation on the protein
  • Characterizing the glycan structure
  • Protein trafficking
11123MA
Recombinant PNGase F deglycosylates multiple substrates.

Twenty micrograms of various denatured glycoprotein standards were treated with 10 units of Promega recombinant or native PNGase F for 1 hour at 37°C. Gel shifts indicating deglycosylation were monitored by SDS-PAGE.

Schematic illustrating the use of PNGase F and mass spec analysis of N-glycosylation.

Protocols

Specifications

What's in the box?

Item Part # Size Concentration

PNGase F

V483A 1 × 500 units 10u/μl

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

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