Glycosidases for Mass Spectrometry
Endoglycosidases and exoglycosidases are tools for structural and functional analysis of glycoproteins. Endoglycosidases like PNGase F and Endo H are routinely used to remove carbohydrates from N-glycosylated proteins. We recommend a mixture of endoglycosidases and exoglycosidases to analyze O-glycosylated proteins.
Promega products for studying protein glycosylation include PNGase F, Endonuclease H and Fetuin. Fetuin is a glycoprotein that can be used as a positive control in glycosylation analysis.
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