Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

rAsp-N, Mass Spec Grade

Click here to customize this product

Hydrolyzes Peptide Bonds N-Terminal to Aspartic Acid Residues

  • Less expensive alternative to native Asp-N
  • Larger volume (5X more protease) for more consistent resuspension
  • Use in complex proteomic analyses and peptide mapping of purified proteins


Catalog number selected: VA1160

$ 546.00
Your price:
Please Enquire
This product is discontinued
Add to Helix
rAsp-N, Mass Spec Grade
$ 546.00
Your price: Log in
Overview 3d image for rAspN protease

Asp-N, Now Available in Recombinant Form

rAsp-N, Mass Spec Grade, is a recombinant protease that was cloned from Stenotrophomonas maltophilia and purified from E. coli. rAsp-N is a highly active protease suitable for proteomic analysis of complex mixtures as well as peptide mapping of purified proteins, such as therapeutic monoclonal antibodies. The protease is provided in 10µg aliquots in a conical vial for easy and consistent resuspension.

Efficient Protein Digestion in 1 Hour

Images shows rAsp-N digests N-terminal to aspartic acid residues.
Recombinant Asp-N provides efficient digestion of a yeast extract in 1 hour. Digestion efficiency was approximately 85% after 1 hour and increased only slightly (to approximately 91%) after overnight digestion.

Digestion N-Terminal to Aspartic Acid Residues

Image shows digestion efficiency of rAsp-N.
rAsp-N, Mass Spec Grade, digests yeast extract proteins primarily N-terminal to aspartic acid residues. Weak digestion at glutamic acid is observed in some cases.

Peptide Mapping with rAsp-N or Trypsin/Lys-C

Peptide mapping with rAsp-N and Trypsin/Lys-C.
Base peak chromatograms showing digestion of NISTmAb for 18 hours with rAsp-N (Panel A; 50:1 w:w) or with Trypsin/Lys-C (Panel B; 25:1 w:w). Digests were analyzed with a Q Exactive™ Plus mass spectrometer (Thermo Fisher Scientific). Sequence coverage from each digest is shown in the table (Panel C).


What's in the box?

Item Part # Size

rAsp-N, Mass Spec Grade

VA116A 1 × 10μg


Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


Let's find the product that meets your needs.

Talk to a Scientist