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rLys-C, Mass Spec Grade

Recombinant Lys-C Cleaves at C-Terminus of Lysines

  • Optimal activity in the pH range of 8-9
  • Retains activity in denaturing conditions such as 8M urea
  • Available lyophilized or frozen

Size

Catalog number selected: V1671

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rLys-C, Mass Spec Grade
15μg
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rLys-C, Mass Spec Grade, is a recombinant Lys-C expressed in E. coli. Sequence origin of rLys-C is Protease IV from Pseudomonas aeruginosa. Similar to a native Lys-C, rLys-C cleaves at the carboxyl side of lysine residues with exceptional specificity. rLys-C retains proteolytic activity under protein denaturing conditions such as 8M urea, which is used to improve digestion of proteolytically resistant proteins. rLys-C activity is optimal in the pH range of 8–9. The protease is supplied in a lyophilized form along with a Reconstitution Buffer, which is formulated to increase stability of rLys-C solution. Frozen rLys-C solution can be stored for a month at –20ºC without detectable loss of activity. rLys-C is recommended for digestion of single proteins and complex protein mixtures in-solution and in-gel.

rLys-C has been shown to be free of contaminating peptides by reverse-phase HPLC. This recombinant product is severalfold less expensive than, yet retains the activity of native rLys-C.

Applications

  • Peptide mapping
  • Protein identification
8634ME.eps
Cut sites for Arg-C, Sequencing Grade; Glu-C, Sequencing Grade; Asp-N, Sequencing Grade; Trypsin Gold, Mass Spectrometry Grade; Sequencing Grade Modified Trypsin; Immobilized Trypsin; Chymotrypsin, Sequencing Grade; Endoproteinase Lys-C, Sequencing Grade; rLys-C, Mass Spec Grade.

Protocols

Specifications

What's in the box?

Item Part # Size

rLys-C, Mass Spec Grade

V167A 1 × 15μg

Resuspension Buffer

V181A 1 × 500μl

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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