Common shortcomings of protein mass spec sample preparation include incomplete digestion, lengthy and laborious procedure, low protein sequence coverage, inefficient peptide recovery of in-gel digested proteins or poor protein extraction from cells and tissues and inadequate instrument performance monitoring. In this webinar, we offer solutions to these shortcomings and discuss the advantages of trypsin preparations with enhanced proteolytic efficiency. We also review mass spec compatible SDS surfactant analogs and their advantage for in-gel protein digestion, peptide recovery and protein extraction from tissues. The discussion includes a case study showing positive impact of proteases with alternative cleavage specificity for increasing of sequence coverage of analyzed proteins. In the final part, we review the advantages of standardized total cell protein extracts and a mix of heavy isotope labeled peptide standards for optimization of protein mass spec sample preparation and monitoring of key performance parameters of mass spec instruments.
Sergei Saveliev, PhD
Senior Research Scientist
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