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ProteaseMAX™ Surfactant, Trypsin Enhancer

V2071o

Improved In-Gel and In-Solution Protein Digestion

  • Enhances enzymatic performance of trypsin, chymotrypsin and Lys-C
  • Saves time and labor when used with in-gel protein digestion
  • Complete digestion in 1 hour

Size

Catalog number selected: V2071

$ 63.00 Your price: Log in

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ProteaseMAX™ Surfactant, Trypsin Enhancer
1mg
$ 63.00
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ProteaseMAX™ Surfactant ensures fast and efficient protein digestion with proteases such as Trypsin, Chymotrypsin and Lys-C. For in-gel protein digestion, ProteaseMAX™ Surfactant offers time and labor savings. Digestion step is complete in 1 hour, and the surfactant provides concurrent extraction of peptides from gels, eliminating the need for post-digestion peptide extraction. The surfactant also improves recovery of longer peptides that are retained in the gel under a standard extraction protocol.

For in-solution digestions, ProteaseMAX™ Surfactant solubilizes proteins, including difficult proteins (i.e., membrane proteins), and enhances protein digestion by providing a denaturing environment prior to protease addition.

ProteaseMAX™ Surfactant degrades over the course of a digestion reaction, yielding products that are compatible with downstream methods such as mass spectrometry (MS) and liquid chromatography (LC). No long-term negative effect of the residual surfactant on the ion optics and capillary of mass spectrometers has been observed. ProteaseMAX™ Surfactant can be used with existing in-gel or in-solution digestion protocols.

Applications

  • In-gel and in-solution protein digestion
  • Concurrent peptide extraction from gels

Benefits of ProteaseMAX™ Surfactant for In-Gel Digestion

11253MA-W

ProteaseMAX™ Surfactant Improves Trypsin Access

11228MA-W

Schematic illustrating the enhanced digestion of proteins with ProteaseMAX™ Surfactant.

ACS Article Features Mass Spec-Compatible Surfactant in Enhanced In-Gel Digestion

This article demonstrates that the use of a particular mass spec-compatible surfactant effectively addresses the challenges of in-gel protein digestion. Mass Spectrometry Compatible Surfactant for Optimized In-Gel Protein Digestion. Saveliev et al. (2013) Anal. Chem. 85, 907.

Read the Article

Specifications

You are viewing: V2071 Change Configuration

What's in the box?

Item Part # Size

ProteaseMAX™ Surfactant, lyophilized

V207A 1 × 1mg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: V2072 Change Configuration

What's in the box?

Item Part # Size

ProteaseMAX™ Surfactant, lyophilized

V207A 5 × 1mg

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

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