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Endoglycosidase H

Characterize Protein Glycosylation

  • Monitor protein trafficking
  • Characterize glycan location and structure
  • Does not cleave complex glycans
  • Supplied at a concentration of 500u/μl

Size

Catalog number selected: V4871

$ 90.00
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Endoglycosidase H
10,000 units
$ 90.00
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Characterize Protein Glycosylation with Endoglycosidase H

Endoglycosidase H (Endo H) is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli. Endo H cleaves the chitobiose core of high mannose and a limited number of hybrid oligosaccharides from N-linked glycoproteins. It does not cleave complex glycans. Enzymatic cleavage is between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, leaving one N-acetylglucosamine residue on the asparagine. This is in contrast to PNGase F, which cleaves all asparagine-linked oligosaccharides.

Unit Definition: One unit is defined as the amount of enzyme required to remove >95% of the carbohydrate from 10μg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10μl.

Applications
  • Determining the state of protein glycosylation
  • Characterizing glycan structure
References
  1. Maley, F. et al. (1989) Anal. Biochem. 180, 195–204.

References using Endo H to monitor protein trafficking:

  1. Taner, S. et al. (2011) J. Immunol. 186, 62–72.
  2. Beyer, S. et al. (2011) Endo. 152, 782–92.
  3. Chen, Z. et al. (2011) J. Exper. Med. 208, 2633–40.
  4. Iavarone, C. et al. (2011) J. Immunol. 186, 4213–22.
  5. Guo, Y. et al. (2011) J. Exper. Med. 208, 2083–98.

Specifications

You are viewing: V4871 Change Configuration

What's in the box?

Item Part # Size
Endo H V487A 1 × 10,000 units
10X Endo H Reaction Buffer V490A 1 × 1ml
10X Denaturing Solution V492A 1 × 1ml

SDS

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Download SDSPDF (101 KB) – English (United States)

Certificate of Analysis

See all certificates for V4871

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: V4875 Change Configuration

What's in the box?

Item Part # Size
Endo H V487B 1 × 50,000 units
10X Endo H Reaction Buffer V490A 1 × 1ml
10X Denaturing Solution V492A 1 × 1ml

SDS

Choose language:

Download SDSPDF (101 KB) – English (United States)

Certificate of Analysis

See all certificates for V4875

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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