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IdeS Protease and IdeZ Protease

Antibody Fragmentation and Characterization, Simplified

  • Highly specific IgG cleavage
  • Rapid generation of highly pure fragments
  • Frozen protease is supplied at a concentration of 50u/µl

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Size

Catalog number selected: V7511

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IdeS Protease and IdeZ Protease
Lyophilized/IdeS 5,000 units
$ 892.00
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32609757-L1-IdeS-IdeZ-Background-2

IgG-Digesting Enzymes for Therapeutic Antibody Characterization

IdeS and IdeZ Protease are immunoglobulin G (IgG)-degrading enzymes that are valuable tools for the characterization of therapeutic antibodies, Fc fusion proteins and antibody-drug conjugates.

IdeS and IdeZ Proteases are fast, easy-to-use proteases; complete digestion  can be achieved in 30 minutes without optimization. Cleavage is highly reproducible and specific, at a single site below the hinge region.

Both IdeS and IdeZ Proteases effectively cleave human IgG1, IgG2, IgG3 and IgG4, monkey, sheep, rabbit, humanized and chimeric IgGs as well as Fc-fusion proteins. However, only IdeZ Protease cleaves mouse IgG2a and IgG3.

32609757-L1-IdeS-IdeZ-Foreground-2

Cleavage Specificity of IdeS and IdeZ Proteases

12335MB-W

IdeS and IdeZ Proteases are immunoglobulin G (IgG)-degrading proteases that cleave with high specificity.

IdeS Protease is derived from Streptococcus pyogenes. It is an engineered, recombinant protease overexpressed in E. coli. IdeS Protease cleaves IgG at a single site below the hinge region, yielding F(ab')2 and Fc fragments as shown here.

IdeZ Protease is derived from Streptococcus equi subspecies zooepidemicus. It is also an engineered, recombinant protease overexpressed in E. coli. Like IdeS, IdeZ Protease specifically cleaves IgG molecules below the hinge region, yielding F(ab')2 and Fc fragments. However, IdeZ Protease has significantly improved activity against mouse IgG2a and IgG3 subclasses compared to IdeS Protease.


Core and Lower Hinge Sequence of Human and Mouse IgG Subclasses

IdeS and IdeZ Protease activity against each subclass is indicated. Cut sites are indicated by bolded letters.
SubclassHinge/CH2 SequenceIdeS ActivityIdeZ Activity
Human
IgG1
CPPCPAPELLGGPSVF
+++
+++
IgG2
CPPCPAPP_VAGPSVF
+++
+++
IgG3
CPRCPAPELLGGPSVF
+++
+++
IgG4
AHHAQAPEFLGGPSVF
+++
+++
SubclassHinge/CH2 SequenceIdeS ActivityIdeZ Activity
Mouse
IgG1
PCICTVPEV_SSVF
IgG2a
CPPCAAPNLLGGPSVF
+
+++
IgG2b
CHKCPAPNLEGGPSVF
IgG3
GSSCPAGNILGGPSVF
+
+++

Preparation of F(ab′)₂ Fragments using IdeS Protease and Magne™ Protein A Beads

12337MA-W-a-2
Panel A. Generation of F(ab')2 fragments using IdeS Protease and Magne™ Protein A Beads.
12337MA-W-b-2
Panel B. Gels demonstrating separation of F(ab')2 and Fc fragments from three antibodies. Fc fragments were captured using Magne™ Protein A beads.

Specifications

You are viewing: V7511 Change Configuration

What's in the box?

Item Part # Size

IdeS Protease

V751A 1 × 5,000 units

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Effective June 29, 2016, a settlement between Promega Corporation and Genovis AB maintains full freedom for continued offering of Promega’s IdeS and IdeZ Proteases for markets worldwide.

Specifications

You are viewing: V7515 Change Configuration

What's in the box?

Item Part # Size

IdeS Protease

V751A 5 × 5,000 units

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Effective June 29, 2016, a settlement between Promega Corporation and Genovis AB maintains full freedom for continued offering of Promega’s IdeS and IdeZ Proteases for markets worldwide.

Specifications

You are viewing: V7512 Change Configuration

What's in the box?

Item Part # Size

IdeS Protease, Frozen

V751B 1 × 2,000 units

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

Effective June 29, 2016, a settlement between Promega Corporation and Genovis AB maintains full freedom for continued offering of Promega’s IdeS and IdeZ Proteases for markets worldwide.

Specifications

You are viewing: V8341 Change Configuration

What's in the box?

Item Part # Size

IdeZ Protease

V834A 1 × 5,000 units

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

GG

Patents and Disclaimers

Effective June 29, 2016, a settlement between Promega Corporation and Genovis AB maintains full freedom for continued offering of Promega’s IdeS and IdeZ Proteases for markets worldwide.

Specifications

You are viewing: V8345 Change Configuration

What's in the box?

Item Part # Size

IdeZ Protease

V834A 5 × 5,000 units

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

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View more results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

GG

Patents and Disclaimers

Effective June 29, 2016, a settlement between Promega Corporation and Genovis AB maintains full freedom for continued offering of Promega’s IdeS and IdeZ Proteases for markets worldwide.

Specifications

You are viewing: V8342 Change Configuration

What's in the box?

Item Part # Size

IdeZ Protease, Frozen

V834B 1 × 2,000 units

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

GG

Patents and Disclaimers

Effective June 29, 2016, a settlement between Promega Corporation and Genovis AB maintains full freedom for continued offering of Promega’s IdeS and IdeZ Proteases for markets worldwide.

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