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Glu-C, Sequencing Grade

Cleaves at the C-Terminus of Either Aspartic or Glutamic Acid Residues

  • Optimal activity at pH 4–9
  • Ammonium and phosphate buffers can affect cleavage; see details in product information
  • Suitable for in-solution digestion; not recommended for in-gel digestion

Size

Catalog number selected: V1651

$ 157.00 Your price: Log In

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Glu-C, Sequencing Grade
50μg
$ 157.00
Your price: Log In

This sequencing grade serine protease (S. aureus V8) specifically cleaves at the C-terminus of either aspartic or glutamic acid residues. In ammonium bicarbonate and ammonium acetate the enzyme specificity is higher at the glutamic residues. In phosphate buffers cleavage occurs at the aspartic and glutamic residues. Glu-C activity is optimal in the pH range of 4.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in solution but not recommended for in-gel digestions. (S. aureus V8)

Applications
  • Peptide mapping
  • Protein identification
8634ME.eps
Cut sites for Arg-C, Sequencing Grade; Glu-C, Sequencing Grade; Asp-N, Sequencing Grade; Trypsin Gold, Mass Spectrometry Grade; Sequencing Grade Modified Trypsin; Immobilized Trypsin; Chymotrypsin, Sequencing Grade; Endoproteinase Lys-C, Sequencing Grade; rLys-C, Mass Spec Grade.

Protocols

Specifications

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Item Part # Size

Glu-C, Sequencing Grade

V165A 5 x 10μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

EE

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