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Subcloning Notebook

Subcloning is a basic molecular biology procedure used to move inserts from one vector to another. The Subcloning Notebook leads you through each step in the process, from digestion of the parent vector, through purification of the insert to ligation and transformation into competent cells.

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Guide Contents

Classic Subcloning
PDF (779kb)

PCR Subcloning
PDF (472kb)

Transforming Bacteria
PDF (352kb)

Screening for Recombinants
PDF (415kb)

Technical Appendix
PDF (256kb)