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T4 DNA Polymerase

Synthesizes DNA 5´→3´ from a Primed ssDNA Template

  • High fidelity enzyme of choice for applications where misincorporation is a concern
  • Flexible polymerase used in a variety of molecular applications; active in many Promega 1X restriction enzyme buffers
  • Heat inactivated by heating at 75°C for 10 minutes
  • Supplied at a concentration of 5–10u/μl

Size

Catalog number selected: M4211

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T4 DNA Polymerase
100u
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T4 DNA Polymerase catalyzes the 5´→3´ synthesis of DNA from a primed single-stranded DNA template. Although possessing a potent 3´→5´ proofreading exonuclease, T4 DNA Polymerase contains no 5´→3´ exonuclease activity. T4 DNA Polymerase can be used to fill in 5´ protruding ends with labeled or unlabeled dNTPs or for the generation of blunt ends from DNA molecules with 3´ overhangs or in vitro mutagenesis. The enzyme is provided with 10X Reaction Buffer: 250mM Tris-acetate (pH 7.7), 1M potassium acetate, 100mM magnesium acetate and 10mM DTT.

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

References

  1. Challberg, M.D. and Englund, P.T. (1980) Meth. Enzymol. 65, 39–43.
  2. Burd, J.F. and Wells, R.D. (1974) J. Biol. Chem. 249, 7094–801.
  3. Kunkel, T.A., Loeb, L.A. and Goodman, M.F. (1984) J. Biol. Chem. 259, 1539–45.

Storage Buffer: 200mM potassium phosphate (pH 6.5 at 25°C), 2mM DTT and 50% glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, SDS-PAGE/purity, endonuclease.

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 55nmol of dTTP into acid-precipitable material in 30 minutes at 39°C using poly(dA):oligo(dT) as a substrate.

Protocols

Specifications

You are viewing: M4211 Change Configuration

What's in the box?

Item Part # Size

T4 DNA Polymerase

M421A 1 × 100u

T4 DNA Polymerase 10X Reaction Buffer

M831A 1 × 1ml

SDS

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Certificate of Analysis

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For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: M4215 Change Configuration

What's in the box?

Item Part # Size

T4 DNA Polymerase

M421F 1 × 500u

T4 DNA Polymerase 10X Reaction Buffer

M831A 1 × 1ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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