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Alkaline Phosphatase, Calf Intestinal (CIAP)

Catalyzes the Hydrolysis of 5´ Phosphate Groups from DNA, RNA, and r- and dNTPs

  • Available at high concentration; Cat.# M2825 contains 1,000 units of CIAP at 20u/μl
  • Qualified for blue/white cloning; our assay provides a higher level of quality control for enzymes used in cloning applications

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Catalog number selected: M1821

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Alkaline Phosphatase, Calf Intestinal (CIAP)
1u/µl/1,000u
$ 81.00
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Alkaline Phosphatase, Calf Intestinal (CIAP), catalyzes the hydrolysis of 5´-phosphate groups from nucleic acids and nucleotide triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vector DNA by removing phosphate groups from both 5´-termini and may also be used for the dephosphorylation of 5´ phosphorylated ends of DNA or RNA for subsequent labeling with [32P]ATP and T4 Polynucleotide Kinase. CIAP is active on 5´ overhangs, 5´ recessed and blunt ends. CIAP is provided with 10X Reaction Buffer: 0.5M Tris-HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM spermidine.

Applications

  • Preventing religation of linearized cloning vehicle DNA by removing phosphate groups from both 5´ termini.
  • Removing 5´ phosphate groups prior to end-labeling with T4 Polynucleotide Kinase.

References

  1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
  2. Seeburg, P.H. et al. (1977) Nature 270, 486–94.
  3. Ullrich, A. et al. (1977) Science 196, 1313–9.
  4. Meyerowitz, E.M. et al. (1980) Gene 11, 271–82.
  5. Grosveld, F.G. et al. (1981) Gene 13, 227–37.

Storage Buffer: 10mM Tris-HCl (pH 8.0), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% (v/v) glycerol.

Source: Calf intestinal mucosa.

QC Tests: Activity, endonuclease, DNase, RNase, blue/white cloning assay.

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1μmol of 4-nitrophenyl phosphate per minute at 37°C in 1M diethanolamine, 10.9mM 4-nitrophenyl phosphate, 0.5mM MgCl2 (pH 9.8 at 25°C).

Protocols

Specifications

You are viewing: 1,000u Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Alkaline Phosphatase, Calf Intestinal

M182A 1 x 1,000u

Alkaline Phosphatase 10X Buffer

M183A 2 x 500μl View Product

SDS

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Certificate of Analysis

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For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: 1,000u Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Alkaline Phosphatase 10X Buffer

M183A 2 x 500μl View Product

Alkaline Phosphatase Calf Intestinal (HC)

M282A 1 x 1,000u

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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