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T4 DNA Ligase

Joins Two DNA Strands with Cohesive or Blunt Ends

  • Use with 5´, 3´ or blunt-ended dsDNA (e.g., inserts and vectors)
  • Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications
  • Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule

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Catalog number selected: M1801

$ 49.00
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T4 DNA Ligase
1–3u/µl/100u (Weiss units)
$ 49.00
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T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The enzyme will not join single-stranded nucleic acids. T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP.

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Reference

  1. Engler, M.J. and Richardson, C.C. (1982) In: The Enzymes, Boyer, P.D., ed., Academic Press, New York, NY.

Note: Units listed are Weiss units.

Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.

Unit Definition: 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.

Specifications

You are viewing: M1801 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

T4 DNA Ligase 10X Buffer

C126B 1 × 200μl View Product

T4 DNA Ligase

M180A 1 × 100u

SDS

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Download SDSPDF (67 KB) – English (United States)

Certificate of Analysis

See all certificates for M1801

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Specifications

You are viewing: M1804 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

T4 DNA Ligase 10X Buffer

C126A 2 × 500μl View Product

T4 DNA Ligase

M180B 1 × 500u

SDS

Choose language:

Download SDSPDF (67 KB) – English (United States)

Certificate of Analysis

See all certificates for M1804

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Specifications

You are viewing: M1794 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

T4 DNA Ligase 10X Buffer

C126B 1 × 200μl View Product

T4 DNA Ligase (HC)

M179A 1 × 500u

SDS

Choose language:

Download SDSPDF (67 KB) – English (United States)

Certificate of Analysis

See all certificates for M1794

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

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