Notes: GAL4-recombinant desmoplakin (DP) domain constructs were immunoprecipitated from yeast extracts. Some immunoprecipitates were treated with Promega’s Calf Alkaline Phosphatase (CIAP). The immunoprecipitates were then analyzed by Western blotting with an anti-GAL4 antibody.  A change in the mobility of Gal4-DP was observed after CIAP treatment. (3263)

Notes: The authors used Calf Intestinal Alkaline Phosphatase to treat membrane preparations from either control- or TNFα-treated human polymorphonuclear neutrophils. Dephosphorylation of protein (Acetyl-CoA:1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine acetyltransferase) in microsome preps by calf intestinal alkaline phosphatase ablates increase in activity following TNFα treatment. (0623)

Notes: The Calf Intestinal Alkaline Phosphatase (CIAP) was used to 5´-dephosphorylate in vitro transcribed RNAs prior to end labeling. (0989)

Notes: Calf Intestinal Alkaline Phosphatase was used for in vitro dephosphorylation of the RAP46 protein, and the dephosphorylated protein was assayed for interaction with various transcription factors. The TNT^® Coupled Reticulocyte Lysate System was used to produce the transcription factors. The Rabbit Reticulocyte Lysate was used to aid protein refolding of heat denatured luciferase and the extent of refolding was examined with the Luciferase Assay System. (0084)