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DeadEnd™ Colorimetric TUNEL System

A Modified TUNEL Assay for Detection of Apoptotic Cells In Situ

  • Detects apoptosis in tissue sections or cell cultures

Size

Catalog number selected: G7360

$ 400.00 Your price: Log In

DeadEnd™ Colorimetric TUNEL System
20 reactions
$ 400.00
Your price: Log In
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The DeadEnd™ Colorimetric TUNEL System is a modified TUNEL Assay that provides simple, accurate and rapid detection of apoptotic cells in situ at the single-cell level. The assay measures nuclear DNA fragmentation, an important biochemical indicator of apoptosis, and can be used to detect apoptotic cell death in tissue sections and cultured cells. The fragmented DNA of apoptotic cells is end-labeled using a modified TUNEL (TdT-mediated dUTP Nick-End Labeling) assay. Biotinylated nucleotide is incorporated at the 3´-OH DNA ends using Terminal Deoxynucleotidyl Transferase (TdT). Horseradish-peroxidase-labeled streptavidin (Streptavidin HRP) is then bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and the stable chromogen, diaminobenzidine (DAB). Using this procedure, apoptotic nuclei are stained dark brown.

Features and Benefits

  • Detects apoptosis in thick tissue sections or assess cell morphology.
  • Assay includes DAB substrate and H2O2 for color detection and plastic coverslips that simplify sample handling.
  • Proven applications include Vibratome® sections of neuronal tissue, Jurkat cells, HL-60 cells.

Note: The protocol for the DeadEnd™ TUNEL Assay recommends an optional DNase I treatment of samples as a positive control to detect DNA fragmentation. RQ1 RNase-Free DNase (Cat.#M6101) can be used to generate the positive control and is available separately.

Protocols

Specifications

You are viewing: G7360 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Plastic Coverslips

G326B 1 × 20 each

Equilibration Buffer

G327B 1 × 4.8ml

SSC, 20X

G329B 1 × 20ml

Streptavidin HRP

G714A 1 × 40μl

Biotinylated Nucleotide Mix

G715A 1 × 20μl

DAB 20X Chromogen

G716A 1 × 200μl

DAB Substrate 20X Buffer

G717A 1 × 200μl

Hydrogen Peroxide 20X

G718A 1 × 200μl

Terminal Deoxynucleotidyl Transferase (recombinant)

M828B 1 × 600u

Proteinase K

V302A 1 × 10mg View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Specifications

You are viewing: G7130 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Plastic Coverslips

G326B 2 × 20 each

Equilibration Buffer

G327C 1 × 9.6ml

SSC, 20X

G329A 1 × 70ml

Streptavidin HRP

G714A 1 × 40μl

Biotinylated Nucleotide Mix

G715A 2 × 20μl

DAB 20X Chromogen

G716A 1 × 200μl

DAB Substrate 20X Buffer

G717A 1 × 200μl

Hydrogen Peroxide 20X

G718A 1 × 200μl

Terminal Deoxynucleotidyl Transferase (recombinant)

M828B 2 × 600u

Proteinase K

V302A 1 × 10mg View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Resources

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