The DeadEnd™ Colorimetric TUNEL System is a modified TUNEL Assay that provides simple, accurate and rapid detection of apoptotic cells in situ at the single-cell level. The assay measures nuclear DNA fragmentation, an important biochemical indicator of apoptosis, and can be used to detect apoptotic cell death in tissue sections and cultured cells. The fragmented DNA of apoptotic cells is end-labeled using a modified TUNEL (TdT-mediated dUTP Nick-End Labeling) assay. Biotinylated nucleotide is incorporated at the 3´-OH DNA ends using Terminal Deoxynucleotidyl Transferase (TdT). Horseradish-peroxidase-labeled streptavidin (Streptavidin HRP) is then bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and the stable chromogen, diaminobenzidine (DAB). Using this procedure, apoptotic nuclei are stained dark brown.
Features and Benefits
- Detects apoptosis in thick tissue sections or assess cell morphology.
- Assay includes DAB substrate and H2O2 for color detection and plastic coverslips that simplify sample handling.
- Proven applications include Vibratome® sections of neuronal tissue, Jurkat cells, HL-60 cells.
Note: The protocol for the DeadEnd™ TUNEL Assay recommends an optional DNase I treatment of samples as a positive control to detect DNA fragmentation. RQ1 RNase-Free DNase (Cat.#M6101) can be used to generate the positive control and is available separately.