We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Have you purchased from Promega in the last six months? Take our brief survey ›

RQ1 RNase-Free DNase

Degrades Single-Stranded or Double-Stranded DNA

  • Produces 3´ hydroxyl oligonucleotides during degradation
  • Preparation qualified for use in applications where maintaining the integrity of RNA is critical
  • Supplied at a concentration of 1u/μl
Click here to customize this product

Introducing the fastest way to order everything you need.

Learn more ›

Size

Catalog number selected: M6101

$ 51.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
RQ1 RNase-Free DNase
1,000u
$ 51.00
Your price: Log in

RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. The enzyme is provided with 10X Reaction Buffer (400mM Tris-HCl [pH 8.0 at 25°C], 100mM MgSO4, 10mM CaCl2) and Stop Buffer (20mM EGTA [pH 8.0 at 25°C])

Applications

  • Preparation of DNA-free RNA.
  • Degradation of DNA from RNA transcription systems.
  • Nick translation of DNA.
  • Studying DNA:protein interactions by DNase I footprinting.

Storage Buffer: 10mM HEPES (pH 7.5 at 25°C), 50% (v/v) glycerol, 10mM CaCl2, 10mM MgCl2.

Source: Bovine pancreas.

QC Tests: Activity, RNase.

Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1μg of DNA in 10 minutes at 37°C in 50μl of a buffer containing 40mM Tris-HCl (pH 7.9 at 25°C), 10mM NaCl, 6mM MgCl2, 10mM CaCl2.

Specifications

What's in the box?

Item Part # Size

RQ1 DNase 10X Reaction Buffer

M198A 1 × 1ml

RQ1 DNase Stop Solution

M199A 1 × 1ml

RQ1 RNase-Free DNase

M610A 1 × 1,000u

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for M6101

Search

View more results

No results

Loading…

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-stephen

Stephen

UK