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RQ1 RNase-Free DNase

Degrades Single-Stranded or Double-Stranded DNA

  • Produces 3´ hydroxyl oligonucleotides during degradation
  • Preparation qualified for use in applications where maintaining the integrity of RNA is critical
  • Supplied at a concentration of 1u/μl

Size

Catalog number selected: M6101

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RQ1 RNase-Free DNase
1,000u
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RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. The enzyme is provided with 10X Reaction Buffer (400mM Tris-HCl [pH 8.0 at 25°C], 100mM MgSO4, 10mM CaCl2) and Stop Buffer (20mM EGTA [pH 8.0 at 25°C])

Applications

  • Preparation of DNA-free RNA.
  • Degradation of DNA from RNA transcription systems.
  • Nick translation of DNA.
  • Studying DNA:protein interactions by DNase I footprinting.

Storage Buffer: 10mM HEPES (pH 7.5 at 25°C), 50% (v/v) glycerol, 10mM CaCl2, 10mM MgCl2.

Source: Bovine pancreas.

QC Tests: Activity, RNase.

Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1μg of DNA in 10 minutes at 37°C in 50μl of a buffer containing 40mM Tris-HCl (pH 7.9 at 25°C), 10mM NaCl, 6mM MgCl2, 10mM CaCl2.

Protocols

Specifications

What's in the box?

Item Part # Size

RQ1 DNase 10X Reaction Buffer

M198A 1 x 1ml

RQ1 DNase Stop Solution

M199A 1 x 1ml

RQ1 RNase-Free DNase

M610A 1 x 1,000u

SDS

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

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