Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

The Role of Cell-Free Rabbit Reticulocyte Expression Systems in Functional Genomics

Michele Arduengo, Elaine Schenborn and Robin Hurst
Promega Corporation

Abstract

In the broad area of functional genomics, that is the global characterization of proteins and their function, cell-free rabbit reticulocyte lysate (RRL) has been used extensively to elucidate the mechanisms of mammalian translation, cotranslational modifications, post-translational modifications and translocation of proteins. More recently, RRL has been used as the workhorse for manufacturing  the proteins engaged in interaction, selection and protein evolution studies from DNA or mRNA libraries either in microarray, display or in vitro expression cloning (IVEC) technologies. This chapter highlights recent functional proteomics applications that use cell-free mammalian RRL.

Related Products