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TNT® Coupled Wheat Germ Extract Systems Technical Bulletin

Instructions for Use of Product(s)
L4130, L4140, L5030

Literature # TB165

The TNT® Coupled Wheat Germ Extract Systems provide one-tube, coupled transcription/translation. The systems greatly simplify the process and reduce the time required to obtain results. Standard wheat germ extract-based translations commonly use RNA synthesized in vitro from a SP6, T3 or T7 RNA polymerase promoter. This entire process requires separate reactions with several steps between each reaction. The TNT® Systems bypass many of these steps by incorporating transcription directly in the translation mix. Additionally, the TNT® System reactions often produce significantly more protein (two- to sixfold) in a 1.5-hour reaction than  standard in vitro wheat germ extract translations using RNA templates.

Summary of Changes
The following change was made to the 3/16 revision of this document:
1. The reaction table in Section 4.B was corrected.

Printed in USA. Revised 3/16.

Experienced User Protocols