TNT® Coupled Wheat Germ Extract Systems Technical Bulletin
Instructions for Use of Product(s)
L4130, L4140, L5030
Literature # TB165
The TNT® Coupled Wheat Germ Extract Systems provide one-tube, coupled transcription/translation. The systems greatly simplify the process and reduce the time required to obtain results. Standard wheat germ extract-based translations commonly use RNA synthesized in vitro from a SP6, T3 or T7 RNA polymerase promoter. This entire process requires separate reactions with several steps between each reaction. The TNT® Systems bypass many of these steps by incorporating transcription directly in the translation mix. Additionally, the TNT® System reactions often produce significantly more protein (two- to sixfold) in a 1.5-hour reaction than standard in vitro wheat germ extract translations using RNA templates.
Summary of Changes
The following changes were made to the 9/25 revision of this document:
1. Updated the cover image and fonts.
2. Removed discontinued Cat.# Y4041, Canine Pancreatic Microsomal Membranes.
3. Made miscellaneous text edits.
4. Removed discontinued Related Products and an expired third-party trademark.
Revised 9/25.