Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

TNT® T7 Quick for PCR DNA Technical Manual

Instructions for Use of Product(s)
L5540

Literature # TM235

The TnT® T7 Quick for PCR DNA is a rapid, convenient, coupled transcription/translation system designed for optimum expression of PCR templates. For most PCR templates, the TnT® T7 Quick for PCR DNA reactions produce up to 5 times more protein than other commercially available kits. The PCR-generated DNA can be used directly from the amplification reaction. PCR products from amplification protocols and commercially available systems (e.g., Promega Access RT-PCR System [Cat.# A1250], Roche Diagnostics High Fidelity and Expand™ Long Template PCR Systems, and Invitrogen Platinum Taq) have been successfully tested. The system will work with any other comparable PCR amplification scheme. The PCR products may be added directly to the TnT® T7 Quick for PCR DNA reaction without purification. To use TnT® T7 Quick for PCR DNA, a PCR fragment containing a T7 promoter is added to the TnT® T7 PCR Quick Master Mix and incubated for 60–90 minutes at 30°C. The synthesized proteins then can be analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) followed by autoradiography or phosphorimaging.

Summary of Change
The following change was made to the 5/17 revision of this document:
1. Removed expired patent statements.

Printed in USA. Revised 5/17

Experienced User Protocols