Triglyceride-Glo™ Assay Technical Manual

Instructions for Use of Product(s)
J3160, J3161

Literature # TM600

The Triglyceride-Glo™ Assay provides a luminescent method for measuring triglycerides in cultured cell lysates and other biological samples such as cell culture medium, serum and tissue homogenates. The assay is ideal for measuring triglyceride accumulation and clearance in normal and pathological conditions. Examples include adipocytes, liver samples or cell culture liver models where excess triglyceride accumulation causes steatosis that progresses to nonalcoholic fatty liver disease (NAFLD) and eventually nonalcoholic steatohepatitis (NASH).

The Triglyceride-Glo™ Assay detects triglyceride levels by measuring glycerol that is released from triglycerides in an enzymatic reaction with a lipase: one molecule of glycerol per molecule of triglyceride. Glycerol is measured in a coupled reaction scheme that links the production of NADH to the activation of a proluciferin that produces light with luciferase. The amount of triglyceride is determined from the difference of glycerol measured in the absence (free glycerol) and presence (total glycerol) of lipase. 

Summary of Changes
The following changes were made to the 5/23 revision of this document:
1. The legend for Figure 3 was updated.
2. Equations were reformatted and miscellaneous text edits made.
3. The font was updated.

Revised 5/23.

Experienced User Protocols