Glycerol-Glo™ Assay Technical Manual
Instructions for Use of Product(s)
J3150, J3151
Literature # TM599
The Glycerol-Glo™ Assay provides a luminescent method for measuring glycerol in cell lysates and other biological samples such as cell culture medium, serum and tissue lysates. Glycerol is primarily measured as the product of lipolysis where it is released from triglycerides. Glycerol is also a substrate or product of a variety of other enzymatic or metabolic processes that can be studied with the Glycerol-Glo™ Assay.
The Glycerol-Glo™ Assay measures glycerol in a coupled reaction scheme that links the production of NADH to the activation of a proluciferin that produces light with luciferase. The Glycerol-Glo™ Assay is part of a bioluminescent metabolite assay platform that offers rapid and sensitive metabolite detection in many sample types compatible with high-throughput applications. The assay can be performed in 96- and 384-well plates, and the detection reagents are added directly to samples without the need for organic extraction, making it amenable to high-throughput applications.
Summary of Changes
The following changes were made to the 5/23 revision of this document:
1. The legend for Figure 3 was updated.
2. Equations were reformatted and miscellaneous text edits made.
3. The font was updated.
Revised 5/23.