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DNA Analysis Promega

Cloning, Subcloning and Transcription Vectors

Find vectors for standard and PCR cloning as well as subcloning. Many of these vectors have T3, T7 or SP6 RNA polymerase promoters or a combination of two promoters for use with in vitro transcription. Inserts can be subcloned between vectors, and several plasmids offer blue/white screening.

14 Products


Cloning Method

Blue White Screening Available

Promoter

Accessories

Shop all Cloning, Subcloning and Transcription Vectors

pUC/M13 Sequencing Primers

Sequence inserts cloned into M13 vectors, pUC plasmids and other lacZ-containing plasmids (e.g.,pGEM®-Z and pGEM®-Zf Vectors).

Q5391, Q5401, Q5421, Q5601

pGEM®-11Zf(+) Vector

Standard cloning vector that can be used for in vitro transcription using the T7 and SP6 promoters and ssDNA production.

P2411

pGEM®-9Zf(–) Vector

Standard cloning vector that can synthesize RNA in vitro using T7 and SP6 RNA polymerase promoters and produce ssDNA.

P2391

pGEM®-3Zf(+/–) Vectors

Standard cloning vectors that contain T7 and SP6 promoters flanking the MCS for in vitro RNA synthesis and can produce circular ssDNA.

P2261, P2271

pGEM®-7Zf(+/–) Vectors

Standard cloning vectors that also serve as templates for in vitro transcription from T7 and SP6 promoters and produce circular ssDNA.

P2371, P2251

pGEM®-5Zf(+) Vector

A standard cloning vector that can be in vitro transcribed from the T7 and SP6 promoters and produce circular ssDNA.

P2241

pSP73 Vector

Standard cloning vector that also can be used for transcription of RNA in vitro using the SP6 and T7 RNA polymerase promoters.

P2221

pSP72 Vector

A standard cloning vector that also can be used for transcription of RNA in vitro with the SP6 and T7 RNA polymerase promoters.

P2191

pGEM®-4Z Vector

Standard cloning vector that can also be used for synthesis of RNA in vitro from SP6 and T7 RNA polymerase promoters.

P2161

pGEM®-3Z Vector

Standard cloning vector that can also synthesize RNA in vitro using the SP6 and T7 RNA polymerase promoters.

P2151

pSP64 Poly(A) Vector

A standard cloning vector that can be used for in vitro transcription from the SP6 promoter and to generate poly(A)+ transcripts in vitro.

P1241

pBR322 Vector

Cloning vector that carries genes for tetracycline and ampicillin resistance. Inserts cloned by restriction enzyme digestion.

D1511

pGEM®-T Vector Systems

Vectors that use T overhangs to clone PCR products, offer blue/white screening and include a control insert, ligase and buffer.

A3600, A3610

pGEM®-T Easy Vector Systems

Vectors that can clone PCR products, use blue/white screening and include a control insert, ligase and 2X Rapid Ligation Buffer.

A1360, A1380

Showing 14 of 14 products

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