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GoTaq® G2 Hot Start Master Mixes

Room-Temperature PCR Setup with High-Performance Master Mix

  • All-in-one master mix; just add template and primers
  • Eliminate nonspecific amplification with a hot start enzyme
  • Use the green master mix for direct-to-gel analysis, and the colorless master mix when post-amplification fluorescence or absorbance analysis is required

Choose a master mix

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Catalog number selected: M7422

$ 57.00
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GoTaq® G2 Hot Start Master Mixes
Green/100 reactions
$ 57.00
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Convenient, Room-Temperature Setup and Higher Performance GoTaq® G2 Enzyme

GoTaq® G2 Hot Start Polymerase is bound to a proprietary antibody that blocks activity at temperatures below 70°C for convenient, room-temperature reaction setup. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94–95°C for two minutes, allowing hot-start PCR following room-temperature reaction assembly. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products. In some cases, hot-start PCR may improve yields. GoTaq® G2 Hot Start Polymerase also exhibits 5´→3´ exonuclease activity.

The GoTaq® G2 Hot Start Master Mixes are ready-to-use mixes containing all necessary components (GoTaq® G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium)—you only need to add primer and template, and go!

The GoTaq® G2 Hot Start Green Master Mix also contains a gel loading dye to facilitate downstream gel analysis. The GoTaq® G2 Hot Start Colorless Master Mix contains no gel loading dye for use when downstream applications require fluorescence or absorbance readings without purification.

pcr-guaranteegray-circlePerformance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

GoTaq® G2 Hot Start Master Mix displays superior sensitivity and robust yield.

GoTaq® G2 Hot Start Master Mix displays superior sensitivity and robust yield.
GoTaq® G2 Hot Start Master Mix displays superior sensitivity and robust yield.

Specifications

You are viewing: M7422 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

GoTaq® G2 Hot Start Green Master Mix

M742A 2 × 1.25ml

Nuclease-Free Water

P119A 2 × 1,250μl View Product

SDS

Choose language:

Download SDSPDF (65 KB) – English (United States)

Certificate of Analysis

See all certificates for M7422

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

Specifications

You are viewing: M7423 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

GoTaq® G2 Hot Start Green Master Mix

M742B 1 × 25ml

Nuclease-Free Water

P119C 1 × 25ml View Product

SDS

Choose language:

Download SDSPDF (65 KB) – English (United States)

Certificate of Analysis

See all certificates for M7423

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

Specifications

You are viewing: M7432 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

GoTaq® G2 Hot Start Colorless Master Mix

M743A 2 × 1.25ml

Nuclease-Free Water

P119A 2 × 1,250μl View Product

SDS

Choose language:

Download SDSPDF (65 KB) – English (United States)

Certificate of Analysis

See all certificates for M7432

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

Specifications

You are viewing: M7433 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

GoTaq® G2 Hot Start Colorless Master Mix

M743B 1 × 25ml

Nuclease-Free Water

P119C 1 × 25ml View Product

SDS

Choose language:

Download SDSPDF (65 KB) – English (United States)

Certificate of Analysis

See all certificates for M7433

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

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