Targeted Protein Degradation
Compound-induced protein degradation exploits small molecule compounds that connect protein targets and E3 ligases facilitating recruitment to the proteasome and endogenous degradation. Two of the more common types of compounds of interest for drug discovery and development are "molecular glues" and proteolysis-targeting chimeras (PROTACs).
A summary of an article in ACS Chem. Biol. demonstrating the use of HiBiT to tag endogenous proteins.
Targeting Proteins for Degradation: Characterizing PROTAC Kinetics and Mode of Action Using Live-Cell Assays
Luminescence-based and energy transfer technologies for live-cell, kinetic characterization of PROTAC compound mechanism of action.
Monitoring Functional Mechanisms of Protein Degradation in Living Cells
A live-cell, luminescence-based technology platform that enables characterization of PROTAC compound mechanism of action using either ectopic or endogenous target expression formats.