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Protein analysis and purification Promega

Protein Expression Kits and Reagents

Protein expression systems optimized for cell-free expression from plasmid DNA, RNA or PCR DNA.

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29 Products

Accessory Reagents


Input Nucleic Acid

Kits and Reagents

Shop all Protein Expression Kits and Reagents

TnT® Starter Bundle

A combination of kits to get you started in cell-free expression and detection. Use for in vitro analysis of protein:protein or protein:nucleic acid interactions, or to verify the ability of your clone to express protein.

L1210, L1215

Canine Pancreatic Microsomal Membranes

Evaluate signal peptide cleavage, membrane insertion, translocation and core glycosylation. Compatible with TnT® Lysate, Rabbit Reticulocyte Lysate and Flexi® Lysate Systems.


T7 Sample System

Optimize gene expression with four in vitro translation test systems that use RNA generated by a T7 promoter.


pCMVTnT™ and pTnT™ Vectors

Convenient expression of cloned genes in vitro or in vivo. pCMVTnT™ Vector contains a CMV enhancer/promoter region, which enables strong constitutive expression in many cell types, while RNA phage promoters enable RNA synthesis in vitro.

L5620, L5610

TnT® T7 Quick for PCR DNA

Coupled transcription/translation system for optimum protein expression from PCR templates.


Rabbit Reticulocyte Lysate Translation System, Nuclease Treated

Identify mRNA species, characterize proteins, investigate transcriptionl/translation control.


Luciferase SP6/T7 Control DNAs

Used as functional controls in TnT® Quick Coupled and TnT® Coupled Transcription/Translation Systems. The Control DNAs contain the gene for luciferase under transcriptional control of a phage RNA T7 or SP6 polymerase promoter and a 30-base pair poly[d(A)/d(T)] tail.

L4741, L4821

pGEM® β-Gal Control DNA

Contains coding sequence for β-galactosidase downstream of an E. coli wildtype lacZ promoter. Use as positive control in the E. coli S30 Extract System for Circular DNA.


TnT® Coupled Reticulocyte Lysate Systems

A single-tube, coupled transcription and translation system.

L4600, L4601, L4610, L4611, L4950, L5010, L5020

Potassium Chloride

2.5M solution to supplement TnT® reactions.


Magnesium Acetate

25mM magnesium acetate.


Luciferase Control RNA

A unique functional control for in vitro translation reactions. An uncapped in vitro-transcribed RNA containing a 30-base poly(A) tail that produces functional luciferase when translated. Control reactions are monitored by a luciferase assay.


Flexi® Rabbit Reticulocyte Lysate System

Translation reaction optimization for Mg2+ and K+ concentrations and the option to add DTT. The endogenous Mg2+ concentration of each lysate batch is stated in the product information to aid in optimizing reactions.


Amino Acid Mixtures

The complete mixture is an aqueous solution containing 1mM each of the 20 essential amino acids, compatible with Flexi® Lysate, TnT® Lysate and standard Rabbit Reticulocyte Lysate Systems, and the Wheat Germ Extract and E. coli S30 Systems. Amino Acid Mixtures are also available without cysteine, methionine and cysteine, leucine or methionine.

L4461, L4471, L5511, L9951, L9961

Wheat Germ Extract

Contains components needed for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors) yet is cleared of endogenous amino acids, mRNA and plant pigments to minimize background translation.


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