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Taq DNA Polymerase Selection Guide

Guidelines to help you choose the right Taq formulation for your needs

Factors to consider when choosing a thermostable polymerase for your PCR include the intended application, enzyme characteristics, fidelity, reaction optimization needs and ease-of-use.

We offer a full range of high-quality Taq DNA polymerase formulations that include optimized enzymes and buffer systems for reliable, robust amplification. Taq DNA polymerase products include hot-start and standard PCR options, master mixes, and customizable buffer systems. We also offer Pfu DNA polymerase for applications requiring higher fidelity amplification.

Review the properties of the various Taq and Pfu formulations using the table below to choose the enzyme best suited to your research needs.

Compare thermostable DNA polymerase features

Applications
Maxwell RSC 48 Instrument Photo
Standard PCR
GoTaq® G2 DNA Polymerase
Maxwell RSC 48 Instrument Photo
Hot Start PCR
GoTaq® G2 Hot Start DNA Polymerase
M4021_GoTaq--Long-PCR-Master-Mix_3
Long Range PCR
GoTaq® Long PCR Master Mix
Maxwell RSC 48 Instrument Photo
High Fidelity PCR
Pfu DNA Polymerase

(not available in all countries)

High throughput

not-available
not-available
not-available
not-available

Long PCR

not-available
not-available
not-available
not-available

Cloning and subcloning

not-available
not-available
not-available
not-available

High-fidelity

not-available
not-available
not-available
not-available

Site-directed mutagenesis

not-available
not-available
not-available
not-available

Template generation for sequencing

not-available
not-available
not-available
not-available

Genotyping

not-available
not-available
not-available
not-available

Multiplex PCR

not-available
not-available
not-available
not-available

Colony PCR

not-available
not-available
not-available
not-available

Fast PCR

not-available
not-available
not-available
not-available

Routine PCR

not-available
not-available
not-available
not-available
Characteristics
Standard PCR
GoTaq® G2 DNA Polymerase
Hot Start PCR
GoTaq® G2 Hot Start DNA Polymerase
Long Range PCR
GoTaq® Long PCR Master Mix
High Fidelity
Pfu DNA Polymerase

(not available in all countries)

 - 3´ exonuclease activity

not-available
not-available
not-available
not-available

 - 5´ exonuclease activity

not-available
not-available
not-available
not-available

Amplicon Size

<5kb

<5kb

<40kb

<10kb

Enzyme type

Recombinant

Recombinant

Recombinant

Native

Product Overhang

3´ A

3´ A

3´ A/ blunt

blunt

Hot Start technology

not-available
not-available
not-available
not-available
Formats
Standard PCR
GoTaq® G2 DNA Polymerase
Hot Start PCR
GoTaq® G2 Hot Start DNA Polymerase
Long Range PCR
GoTaq® Long PCR Master Mix
High Fidelity
Pfu DNA Polymerase

(not available in all countries)

Enzyme with direct loading Buffer

not-available
not-available
not-available
not-available

Buffer with MgCl2

GoTaq® G2 Flexi DNA Polymerase

GoTaq® G2 Hot Start DNA Polymerase

not-available
not-available

Contains MgSO4

Master Mix with direct loading Buffer, dNTPs and enhancers (Green)

not-available
not-available
not-available
not-available

Master Mix without direct loading Buffer, dNTPs and enhancers (Colorless)

not-available
not-available
not-available
not-available
 

Compare DNA polymerase features

Maxwell RSC 48 Instrument Photo
Standard PCR

GoTaq® G2 DNA Polymerase


Applications

High throughput
not-available

Long PCR
not-available

Cloning and subcloning

not-available

High-fidelity

not-available

Site-directed mutagenesis

not-available

Template generation for sequencing

not-available

Genotyping

Included Icon

Multiplex PCR

not-available

Colony PCR

Included Icon

Fast PCR

not-available

Routine PCR

Included Icon

Characteristics

5´ - 3´ exonuclease activity

Included Icon

3´ - 5´ exonuclease activity

not-available

Amplicon Size

<5kb


Enzyme type

Recombinant


Product Overhang

3´ A


Hot Start technology

not-available

Formats

Enzyme with direct loading Buffer

Included Icon

Buffer with MgCl2

Included Icon

Buffer without MgCl2

GoTaq® G2 Flexi DNA Polymerase


Master Mix with direct loading Buffer, dNTPs and enhancers (Green)

GoTaq® G2 Green Master Mix


Master Mix without direct loading Buffer, dNTPs and enhancers (Colorless)

GoTaq® G2 Colorless Master Mix

Learn More
Maxwell RSC 48 Instrument Photo
Hot Start PCR

GoTaq® G2 Hot Start DNA Polymerase


Applications

High throughput
Included Icon

Long PCR
not-available

Cloning and subcloning

not-available

High-fidelity

not-available

Site-directed mutagenesis

not-available

Template generation for sequencing

not-available

Genotyping

Included Icon

Multiplex PCR

Included Icon

Colony PCR

Included Icon

Fast PCR

Included Icon

Routine PCR

Included Icon

Characteristics

5´ - 3´ exonuclease activity

Included Icon

3´ - 5´ exonuclease activity

not-available

Amplicon Size

<5kb


Enzyme type

Recombinant


Product Overhang

3´ A


Hot Start technology

Included Icon

Formats

Enzyme with direct loading Buffer

Included Icon

Buffer with MgCl2

not-available

Buffer without MgCl2

GoTaq® G2 Hot Start DNA Polymerase


Master Mix with direct loading Buffer, dNTPs and enhancers (Green)

GoTaq® G2 Hot Start Green Master Mix


Master Mix without direct loading Buffer, dNTPs and enhancers (Colorless)

GoTaq® G2 Hot Start Colorless Master Mix

Learn More
M4021_GoTaq--Long-PCR-Master-Mix_3
Long PCR Master Mix

GoTaq® Long PCR Master Mix


Applications

High throughput
Included Icon

Long PCR
Included Icon

Cloning and subcloning

Included Icon

High-fidelity

Included Icon

Site-directed mutagenesis

Included Icon

Template generation for sequencing

Included Icon

Genotyping

Included Icon

Multiplex PCR

Included Icon

Colony PCR

Included Icon

Fast PCR

not-available

Routine PCR

not-available

Characteristics

5´ - 3´ exonuclease activity

Included Icon

3´ - 5´ exonuclease activity

Included Icon

Amplicon Size

<40kb


Enzyme type

Recombinant


Product Overhang

3´ A/ blunt


Hot Start technology

Included Icon

Formats

Enzyme with direct loading Buffer

not-available

Buffer with MgCl2

Included Icon

Buffer without MgCl2

not-available

Master Mix with direct loading Buffer, dNTPs and enhancers (Green)

not-available

Master Mix without direct loading Buffer, dNTPs and enhancers (Colorless)

Included Icon
Learn More
Maxwell RSC 48 Instrument Photo
High Fidelity

Pfu DNA Polymerase

(not available in all countries)



Applications

High throughput
not-available

Long PCR
not-available

Cloning and subcloning

Included Icon

High-fidelity

Included Icon

Site-directed mutagenesis

Included Icon

Template generation for sequencing

Included Icon

Genotyping

Included Icon

Multiplex PCR

not-available

Colony PCR

Included Icon

Fast PCR

not-available

Routine PCR

not-available

Characteristics

5´ - 3´ exonuclease activity

Included Icon

3´ - 5´ exonuclease activity

Included Icon

Amplicon Size

<10kb


Enzyme type

Native


Product Overhang

blunt


Hot Start technology

not-available

Formats

Enzyme with direct loading Buffer

not-available

Buffer with MgCl2

not-available

Buffer without MgCl2

Included Icon

Contains MgSO4


Master Mix with direct loading Buffer, dNTPs and enhancers (Green)

not-available

Master Mix without direct loading Buffer, dNTPs and enhancers (Colorless)

not-available
Learn More

What are the Advantages of Hot-Start PCR?

  • Hot start PCR reduces non-specific priming and primer dimer formation
  • Antibody-mediated hot start technology allows room temperature reaction set-up
  • Supports use in areas where room temperature may fluctuate
  • Supports automated platforms setup
  • Short 2-minute initial denature time at 94-95°C activates Taq DNA polymerase for amplification
  • Antibodies have no effect on polymerase performance, so all enzyme features are intact as in the non-hot-start enzyme
  • High specificity