GoTaq® G2 Polymerase is the second generation of GoTaq® products. It comes in a variety of formats designed to provide maximum flexibility, control and convenience.
For superior convenience and improved yield, sensitivity and specificity, choose GoTaq® G2 Hot Start Polymerase, which is bound to a proprietary antibody that blocks activity. Activity is restored during initial denaturation, allowing hot-start PCR. Available as a master mix or standalone enzyme.
GoTaq® G2 Hot Start Polymerase is supplied with 5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer and 25mM MgCl2. The high-performance GoTaq® G2 DNA Polymerase is bound to a proprietary antibody that blocks polymerase activity. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94–95°C for two minutes, allowing hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C for convenient, room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products. In some cases, hot-start PCR may improve yields. GoTaq® G2 Hot Start Polymerase exhibits 5´→3´ exonuclease activity.
Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.