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GoTaq® G2 Hot Start Taq Polymerase

High-Performance Hot Start Taq with Convenient Room-Temperature PCR Setup

  • Hot-start Taq eliminates nonspecific PCR amplification
  • Detects low-copy-number targets
  • Allows optimization of reactions with Flexi Reaction Buffer and separate MgCl2
  • Supplied at a concentration of 5u/μl

Size

Catalog number selected: M7401

$ 60.00
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GoTaq® G2 Hot Start Taq Polymerase
100u
$ 60.00
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Hot Start Taq with Superior Performance, Improved Yield, Greater Sensitivity 

GoTaq® G2 Hot Start Taq is the second generation of GoTaq® products. It comes in a variety of formats designed to provide maximum flexibility, control and convenience.

GoTaq® G2 Hot Start Taq is bound to a proprietary antibody that blocks polymerase activity until the reaction is heated to 94–95°C during initial denaturation. Polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start Taq is advantageous for some amplification targets because it may eliminate or minimize formation of primer-dimer or nonspecific products.  In some cases, hot-start PCR may improve yields. 

GoTaq® G2 Hot Start Taq is available as a master mix or as a standalone enzyme, it is supplied with 5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer and 25mM MgCl2. GoTaq® G2 Hot Start Polymerase exhibits 5´→3´ exonuclease activity.

pcr-guaranteegray-circlePerformance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

Hot start taq polymerse gives hiqher yield and greater sensitivity

GoTaq® G2 Hot Start Taq exhibits higher yields and greater detection sensitivity. The 2.2kb APC target was amplified from decreasing amounts of input gDNA (33ng, 3.3ng and 330pg, left to right for each enzyme) using GoTaq® G2 Hot Start Taq with Green (G) and Colorless (C) buffers and the leading competitors (L1, R, Q, Q+, L2 and L3) according to manufacturers’ recommendations.

Specifications

You are viewing: M7401 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 1 × 750μl25mM View Product

GoTaq® G2 Hot Start Polymerase

M740A 1 × 100u

5X Colorless GoTaq® Flexi Buffer

M890A 1 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 1 × 1ml View Product

SDS

Choose language:

Download SDSPDF (117 KB) – English (United States)

Certificate of Analysis

See all certificates for M7401

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Patents and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

Specifications

You are viewing: M7405 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351H 3 × 1.2ml25mM View Product

GoTaq® G2 Hot Start Polymerase

M740B 1 × 500u

5X Colorless GoTaq® Flexi Buffer

M890A 4 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 4 × 1ml View Product

SDS

Choose language:

Download SDSPDF (117 KB) – English (United States)

Certificate of Analysis

See all certificates for M7405

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Patents and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

Specifications

You are viewing: M7406 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351H 15 × 1.2ml25mM View Product

GoTaq® G2 Hot Start Polymerase

M740B 5 × 500u

5X Colorless GoTaq® Flexi Buffer

M890A 20 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 20 × 1ml View Product

SDS

Choose language:

Download SDSPDF (117 KB) – English (United States)

Certificate of Analysis

See all certificates for M7406

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Patents and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

Specifications

You are viewing: M7408 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351H 60 × 1.2ml25mM View Product

GoTaq® G2 Hot Start Polymerase

M740B 20 × 500u

5X Colorless GoTaq® Flexi Buffer

M890A 80 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 80 × 1ml View Product

SDS

Choose language:

Download SDSPDF (117 KB) – English (United States)

Certificate of Analysis

See all certificates for M7408

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Patents and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

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