GoTaq® G2 Hot Start Taq is the second generation of GoTaq® products. It comes in a variety of formats designed to provide maximum flexibility, control and convenience.
GoTaq® G2 Hot Start Taq is bound to a proprietary antibody that blocks polymerase activity until the reaction is heated to 94–95°C during initial denaturation. Polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start Taq is advantageous for some amplification targets because it may eliminate or minimize formation of primer-dimer or nonspecific products. In some cases, hot-start PCR may improve yields.
GoTaq® G2 Hot Start Taq is available as a master mix or as a standalone enzyme, it is supplied with 5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer and 25mM MgCl2. GoTaq® G2 Hot Start Polymerase exhibits 5´→3´ exonuclease activity.
Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.