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Trypsin/Lys-C Mix, Mass Spec Grade

Improve Protein Digestion with this Mix of Trypsin Gold and rLys-C

  • Use standard overnight digestion, nondenaturing conditions
  • Increased peptide recovery, enhanced protein quantitation, improved reproducibility
  • Tolerant to trypsin-inhibiting contaminants

Size

Catalog number selected: V5071

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Trypsin/Lys-C Mix, Mass Spec Grade
20μg
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Trypsin/Lys-C Mix, Mass Spec Grade: See What You've Been Missing

Using standard digestion conditions, Trypsin/Lys-C Mix improves peptide digestion over trypsin alone, increasing peptide recovery and resulting in better protein quantitation.
TrypsinLysC-Proteins-2

Increased Peptide and Protein Identifications Using a Standard Digestion Protocol

11788ME-W-a
11788ME-W-b
11787MB-W
Incomplete digestion with trypsin was evident after comparing peptide peak intensities in trypsin and Trypsin/Lys-C Mix digests.

Improved Reproducibility

11786MB-W
Improved reproducibility of IgG (Rituximab) digestion when using the Trypsin/Lys-C Mix.

Tolerant To Trypsin-Inhibiting Contaminants

13058MC-W-a
13058MC-W-b
Efficient digestion with Trypsin/Lys-C Mix in the presence of trypsin-inhibiting agents. Yeast protein extract was used as a model system.
Conclusions: Use of Trypsin/Lys-C resulted in fewer missed cleavages and a 20% increase in protein identifications.

Protocols

Specifications

You are viewing: 20μg Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

V181A 1 x 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

V507A 1 x 20μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: 100μg Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

V181A 1 x 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

V507B 1 x 100μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: 100μg Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

V181A 1 x 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

V507A 5 x 20μg

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB
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