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6 × 5 LC-MS/MS Peptide Reference Mix

Peptide Mix and Free Software to Monitor LC/MS Instrument Performance

  • Determine mass spec instrument sensitivity and dynamic range
  • No manual calculations, maximized instrument performance with free PReMiS™ Software

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Catalog number selected: V7491

$ 159.00
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6 × 5 LC-MS/MS Peptide Reference Mix
50μl
$ 159.00
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32609642-6x5Peptide-OverviewImage

A Complete Solution for Monitoring LC and MS Instrument Quality Control and Performance

In combination with an accompanying software tool, the 6 × 5 LC-MS/MS Peptide Reference Mix provides a convenient way to assess LC column performance and MS instrument parameters, including sensitivity and dynamic range.

  • Consists of 30 peptides; 6 sets of 5 isotopologues of the same peptide sequence, differing only in the number of stable, heavy-labeled amino acids incorporated into the sequence
  • Labels are uniform 13C and 15N atoms.
  • Isotopologues are chromatographically indistinguishable; however, since they differ in mass, they are clearly resolved by mass spectrometry.
  • The isotopologues of each peptide are present in a series of tenfold dilutions. This format allows assessment of instrument dynamic range and sensitivity from a single run.

The 6 × 5 LC-MS/MS Peptides are AAA-qualified for accurate monitoring, and are compatible with multiple applications and neat or complex mixtures.



Read an ACS Analytical Chemistry article by J. Beri et al. featuring use of the 6 x 5 Reagent: Reagent for Evaluating Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Performance in Bottom-Up Proteomic Experiments.

13869806-BR-PR-6x5-Peptide-Foreground

Workflow with PReMiS™ Software

The complimentary PReMiS™ Software streamlines routine analysis by:

  • Eliminating  time-consuming manual calculations
  • Ensuring consistent instrument performance over time
  • Allowing you to compare instrument-to-instrument performance over time
This diagram shows a standard workflow using PReMiS™ Software to analyze data generated by the 6 × 5 LC-MS/MS Peptide Reference Mix.
Download PreMis™ Software
Workflow-01

12641MA-W-a
12641MA-W-b

Compatible with Neat and Complex Mixtures

  • Use neat format for standard instrument monitoring
  • Spike peptide mix into complex samples (i.e., human cell extract) to monitor instrument performance with relevant experimental samples
The data here show identification of accurate retention times in neat and in complex samples. Retention times were determined when evaluating neat format (500fmol loaded) and when spiking (1pmol) into human K562 cell extract, using provided PReMiS™ Software.
LC-MS/MS Instrument Parameter and Feature Details
Parameter/FeatureCategoryCalculated by SoftwareDetails
Retention Time
LC
Yes
Time required for peptide being separated to elute from the chromatography column.
Peak Width
LC
Yes
Peak width is an indication of column efficiency.
Base Peak Height
LC
Yes
This measure of the signal response can be correlated to the ionization efficiency of the peptide.
Graphical XIC Analysis
LC
Yes
A graphical XIC (eXtracted Ion Chromatogram) is displayed for all 30 ions for visual confirmation of the detectability of all masses. This is especially important for analysis of the peptide mixture spiked into a complex background.
Lowest Detectable Quantity
MS
Yes
Lowest abundance detected is reported for each peptide sequence.
Linear Fit Analysis (Linear Dynamic Range1)
MS
Yes
*The software does not directly calculate the full dynamic range of the instrument. However, up to 5 logs of dynamic range can be determined directly from the Linear Fit Analysis.
LOD and LOQ2
MS
No
The AAA-qualified peptides allow the mix to confirm instrument LOD and LOQ, the basis for the amount loaded, which then can be used to confirm consistency of detection limits.
Slope and r2 of Sensitivity Curve: Graphical Analysis.
MS
Yes
A plot of the log of peptide amount in moles versus log of peak height is reported to provide information on LOD and LOQ.
Mass Accuracy
MS
Yes
Performance Over Time
Both
Yes
Parameters like LC retention time, peak height and mass accuracy are graphically displayed based on date and time.
Multi-Instrument Comparisons
Both
Yes

Specifications

You are viewing: V7491 Change Configuration

What's in the box?

Item Part # SizeConcentration

6 × 5 LC-MS/MS Peptide Reference Mix

V749A 1 × 50μl0.5pmol/μl

Aliquot Tubes

V750A 1 × 6 each

SDS

Choose language:

Download SDSPDF (42 KB) – English (United States)

Certificate of Analysis

See all certificates for V7491

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 10,247,711 and other patents pending.

Specifications

You are viewing: V7495 Change Configuration

What's in the box?

Item Part # Size

6 × 5 LC-MS/MS Peptide Reference Mix

V749B 1 × 200pmol

Aliquot Tubes

V750A 1 × 6 each

SDS

Choose language:

Download SDSPDF (40 KB) – English (United States)

Certificate of Analysis

See all certificates for V7495

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 10,247,711 and other patents pending.

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