Faster Mass Spec: Same-Day Sample Prep Now a Reality
- Reviewing the drawbacks of current sample preparation protocols
- Defining technical parameters of new rapid digestion protocol
- Comparing sequence coverage and total spectra on simple and complex protein samples
using standard vs rapid digestion protocols
- Presenting case studies using the rapid digestion kit
Preparation of protein samples for mass spec analysis is often the first of several bottlenecks in bottom-up proteomics workflow. Recent LC-MS/MS methods are as short as 10-minutes per run, and data analysis is more streamlined. However, the proteolysis step is often carried out overnight, extending mass spec analysis to 2 days. This bottleneck is challenging when preparing samples for drug metabolism (Pharmacokinetics) studies as well as complex protein samples (i.e., analysis of biomarkers). What if you could shorten digestion times to ~30 minutes, without denaturants or reductants/alkylating agents?
Michael M. Rosenblatt, PhD
R&D Group Leader, Mass Spec
Mike is the leader of the Mass Spec Reagents group at Promega Corporation. He received his B.S. degree from Towson University and PhD from the University of Illinois at Urbana-Champaign. After completing an NIH post-doctoral fellowship at the University of Pennsylvania, he founded the Proteomics Core facility at the Children’s Hospital of Philadelphia. Prior to joining Promega he worked with Thermo-Fisher Scientific where he was a Senior Scientist focusing on the development of reagents for the preparation of samples for biological mass spectrometry.
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