Preparation of protein samples for mass spec analysis is often the first of several bottlenecks in bottom-up proteomics workflow. Recent LC-MS/MS methods are as short as 10-minutes per run, and data analysis is more streamlined. However, the proteolysis step is often carried out overnight, extending mass spec analysis to 2 days. This bottleneck is challenging when preparing samples for drug metabolism (Pharmacokinetics) studies as well as complex protein samples (i.e., analysis of biomarkers). What if you could shorten digestion times to ~30 minutes, without denaturants or reductants/alkylating agents?
Michael M. Rosenblatt, PhD
R&D Group Leader, Mass Spec
We love to talk science.
Sign up to receive monthly invitations to our webinars.