Faster Mass Spec: Same-Day Sample Prep Now a Reality
- Reviewing the drawbacks of current sample preparation protocols
- Defining technical parameters of new rapid digestion protocol
- Comparing sequence coverage and total spectra on simple and complex protein samples
using standard vs rapid digestion protocols
- Presenting case studies using the rapid digestion kit
Preparation of protein samples for mass spec analysis is often the first of several bottlenecks in bottom-up proteomics workflow. Recent LC-MS/MS methods are as short as 10-minutes per run, and data analysis is more streamlined. However, the proteolysis step is often carried out overnight, extending mass spec analysis to 2 days. This bottleneck is challenging when preparing samples for drug metabolism (Pharmacokinetics) studies as well as complex protein samples (i.e., analysis of biomarkers). What if you could shorten digestion times to ~30 minutes, without denaturants or reductants/alkylating agents?
Michael M. Rosenblatt, PhD
R&D Group Leader, Mass Spec
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