Enhanced Protein Mass Spectrometry Analysis with Trypsin/Lys-C Mix Scientific Poster
Part # PS181
Marjeta Urh1, Sergei Saveliev1, Ethan Strauss1, Mike Rosenblatt1, Richard Jones2, Michael Ford2 and Dave Allen2
1Promega Corporation, Madison, WI; 2MS BioWorks, Ann Arbor, MI
Efficient proteolysis is required for protein mass spectrometry analysis. Here we describe a Trypsin/Lys-C mix, which dramatically improves proteolysis:
- The majority of missed cleavages, commonly occurring in trypsin digests, are largely eliminated
- Protease-inhibiting impurities are tolerated
- Proteolytically resistant proteins are efficiently digested
We show here how enhanced proteolysis with Trypsin/Lys-C improves protein mass spectrometry analysis. These benefits include an increase in identified peptides and proteins, higher signal intensity of individual peptides and higher analytical reproducibility.
Printed in USA.