Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

United States

BCAA-Glo™ Assay

Image Coming Soon
Click here to customize this product
Click here to customize this product

Easy, Sensitive BCAA Assay Kit

  • Sensitive BCAA detection across broad linear range (50nM to 50µM)
  • Save time with in-well lysis; no cell collection or centrifugation required
  • Amenable to HTS applications

Size

Catalog number selected: CS306201

Your price:
Add to Cart
This product is discontinued
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
BCAA-Glo™ Assay
5ml
Your price: Log in

Detection of BCAA in Biological Samples

Branched chain amino acids (BCAA; leucine, valine and isoleucine) have important roles in many areas of biology as substrates for protein synthesis, an energy source, and essential signaling molecules. The BCAA-Glo™ Assay is a rapid, sensitive method for BCAA detection based on bioluminescent technology, allowing you to detect the smallest changes of BCAAs in a variety of biological sample types.

How the BCAA-Glo™ Assay Works

How to detect BCAAs using the BCAA-Glo™ Assay.

The BCAA-Glo™ Assay uses leucine dehydrogenase to produce NADH in the presence of BCAA, followed by NADH measurement using a bioluminescent NADH detection technology. The result is a light signal proportional to the starting BCAA concentration in the sample.

Simple Protocol for Various Sample Types

BCAA-Glo™ Assay workflow.

Detecting BCAA using BCAA-Glo™ Assay is simple—just add Detection Reagent to samples and incubate. The luminescent output can be analyzed using a standard plate-reading luminometer. The assay is compatible with cell lysates, media samples, tissue homogenates, plasma and serum samples.

Detect the Smallest Changes in Branched Chain Amino Acids

Bioluminescent detection provides superior sensitivity compared to colorimetric and fluorescent assays.

Linear Range

50nM to ~50μM

Maximum Assay Window (S/B)

≥ 200
Data showing the sensitive detection of BCAAs using the BCAA-Glo™ Assay.

Leucine titration curve. The bioluminescent signal (RLU) is proportional to BCAA content of the sample. Precise concentration of BCAA in a given sample can be determined by running a standard curve with the leucine positive control provided in the kit.

In-Well Detection is Easy and Sensitive

For intracellular BCAA measurements, sample preparation steps are streamlined to enable cell lysis and subsequent BCAA detection in the same well. No cell collection, centrifugation or spin columns are needed. Simply remove medium from plated cells and wash with PBS, and then add acid to lyse cells. The lysate can be assayed directly in the well or after being transferred to a new 96- or 384-well plate.

In-well detection of BCAAs using the BCAA-Glo™ Assay.

A small number of cells is required for in-well BCAA detection. A549 cells were dispensed into wells of a 96-well plate in the indicated quantities in quadruplicate. Following in-well lysis, BCAA content in each sample was measured with BCAA-Glo™ Assay.

Kit Size

  • 5ml kit (sufficient for 100 assays in 96-well plate)

Reaction volumes can be scaled easily to 384-well plates for high-throughput applications.

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Specifications

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Resources

Posters

Other Resources

Similar Products

Glucose Uptake-Glo™ Assay

Non-radioactive assay for measuring glucose uptake.

J1341, J1342, J1343

Glutamate-Glo™ Assay

Quickly measure glutamate from many sample types.

J7021, J7022

Glycogen-Glo™ Assay

Rapid, sensitive method for detection of glycogen in biological samples.

CS1823B01, CS1823B02

Lumit™ Immunoassays for Detection of Metabolic Regulators

Assays to detect insulin or glucagon in cell culture samples with a scalable, no-wash protocol.

Frequently Used With

GloMax® Discover System

High-performance microplate reader for detecting luminescence, fluorescence and absorbance.

GM3000

GloMax® Explorer System

Easy-to-use, flexible multimode detection system.

GM3500, GM3510

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-manos

Manos

UK
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
Estonia
Finland
France
/
Germany
/
Iceland
Italy
/
Luxembourg
Netherlands
Norway
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.