Metabolite-Glo™ Detection System

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Easy Metabolite Detection Customizable to Your Specific Targets

  • Bioluminescent NAD(P)H detection toolkit that can be coupled with a metabolite-specific dehydrogenase to build custom metabolite detection assays
  • For use with cultured cells, media samples, tissue homogenates and serum samples
  • Scalable to 384-well format for HTS applications

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Catalog number selected: J9030

$ 400.00
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Metabolite-Glo™ Detection System
5ml
$ 400.00
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Simple Detection of Metabolites from Diverse Samples

The Metabolite-Glo™ Detection System is a rapid and sensitive approach for detecting NAD(P)H in a variety of biological samples, including cultured cells, media samples, tissue homogenates and serum. This system can be optimized to detect specific metabolites by coupling NAD(P)H detection with a suitable dehydrogenase enzyme (which must be obtained separately).  We provide straightforward instructions for metabolite assay development and optimization in the technical manual.

The Metabolite-Glo™ System provides reagents for a streamlined sample preparation with acid treatment and neutralization steps performed directly in the assay wells. This eliminates the need for traditional methods such as cell collection, centrifugation and spin columns. With its sensitive detection capabilities and liquid formulation, the assay is easily adapted to a high-throughput 384-well format, enabling the efficient analysis of numerous samples simultaneously.

How the Metabolite-Glo™ Detection System Works

Diagram illustrating how the Metabolite-Glo™ Assay works.

The core technology provided in the Metabolite-Glo™ Detection System measures NAD(P)H. To measure a metabolite of interest using this system, you need to identify and source a metabolite-specific dehydrogenase. The dehydrogenase couples metabolite oxidation to NAD(P)+ reduction. The reduced dinucleotides, NAD(P)H, are measured using a luciferase reaction. The amount of light produced by luciferase is proportional to the amount of metabolite in the sample.

Example Protocols for Metabolite Detection

Promega scientists have developed protocols that use the Metabolite-Glo™ Detection System with commercially available dehydrogenases and standards for specific metabolite detection from biological samples:

Validated Protocols for Metabolite Detection

Metabolite Application Note

Isocitrate

Download GFN219

Save Time with a Simple Add-and-Read Protocol

Diagram illustrating the Metabolite-Glo™ Assay protocol workflow.

Reagents for in-well sample preparation are included in the Metabolite-Glo™ Detection System to streamline your workflow and reduce variability.

Accurately Detect the Smallest Changes in Metabolite Levels

Graph showing the linear relationship between luminescence and dehydrogenase concentration.

The NAD(P)H core technology does not discriminate between NADH and NAD(P)H, allowing either NAD-dependent or NADP-dependent dehydrogenases to be used for metabolite detection. The sensitivity and linearity of metabolite detection is defined by the core technology and is within 40nM–25μM.

View our portfolio of complete, ready-to-use metabolite detection kits.

Specifications

You are viewing: J9030 Change Configuration

What's in the box?

Item Part # Size Concentration

Reductase

G884A 1 × 55μl 6mg/ml

Reductase Substrate

G885A 1 × 55μl

Luciferin Detection Solution

J135A 1 × 5ml

NAD

J136B 1 × 275μl

Neutralization Buffer

J153A 1 × 15ml

NADP+

J154B 1 × 100μl

0.6N HCl

J402A 1 × 15ml

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Specifications

You are viewing: J9040 Change Configuration

What's in the box?

Item Part # Size Concentration

Reductase

G884B 1 × 275μl 6mg/ml

Reductase Substrate

G885B 1 × 275μl

Luciferin Detection Solution

J135B 1 × 50ml

NAD

J136C 1 × 1ml

Neutralization Buffer

J153A 1 × 15ml

NADP+

J154C 1 × 500μl

0.6N HCl

J402A 1 × 15ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

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