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Bioluminescent Assays for Glucose and Glutamine Metabolism

Leippe, D. et al.
Promega Corporation
Publication Date: 2016

Abstract

In this paper, the authors describe the application of bioluminescent assays for the high-throughput analysis of two major metabolic pathways in cancer cells: glycolysis and glutaminolysis. They use bioluminescent energy metabolism assays to detect glucose, glucose uptake, lactate, glutamine and glutamate. These highly sensitive assays demonstrated a broad linear range, a wide dynamic range and worked well in high-throughput workflows where they could be performed in multiplex with a real-time viability assay to generate controlled data sets.

How to Cite This Article

Scientific Style and Format, 7th edition, 2006

Leippe, D. et al. Bioluminescent Assays for Glucose and Glutamine Metabolism. [Internet] 2016. [cited: year, month, date]. Available from: https://www.promega.com/resources/pubhub/applications-notes/bioluminescent-assays-for-metabolism/

American Medical Association, Manual of Style, 10th edition, 2007

Leippe, D. et al. Bioluminescent Assays for Glucose and Glutamine Metabolism. Promega Corporation Web site. https://www.promega.com/resources/pubhub/applications-notes/bioluminescent-assays-for-metabolism/ Updated 2016. Accessed Month Day, Year.