Cellular metabolism comprises a dynamic network of pathways that undergo reprogramming in response to external and internal signals. This reprogramming has vital roles in proper cell function and also in many disease states, making cancer metabolism and immuno metabolism important areas for research and drug discovery. Metabolite levels provide valuable information about pathway activity, and plate-based coupled-enzyme reaction assays can provide a rapid method for measuring specific metabolites. However, current plate-based metabolite detection assays can pose challenges especially with sample preparation, reproducibility and throughput.
In this webinar we will discuss new plate-based bioluminescent assays for measuring the key metabolites glucose, lactate, glutamine and glutamate, and demonstrate using these assays to study glycolysis and glutaminolysis, two major fuel-metabolizing pathways. We will discuss factors to consider when designing an experiment, including assay sensitivity and linear range, as well as choice of sample preparation method.
Donna Leippe, PhD
Senior Research Scientist
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