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CellTiter-Glo® Luminescent Cell Viability Assay

A Luminescent Assay to Determine the Number of Viable Cells in Culture

  • A homogeneous "add-mix-measure" format reduces the number of steps
  • Higher level of detection requires fewer cells per assay
  • Process plates consecutively with a very stable luminescent signal
  • Integrates with the MyGlo™ Reagent Reader

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Catalog number selected: G7570

$ 128.00
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CellTiter-Glo® Luminescent Cell Viability Assay
CellTiter-Glo® Assay/10ml
$ 128.00
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CellTiter-Glo® Measures ATP, A Key Biomarker of Cell Health

The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. The CellTiter-Glo® Assay is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium and multiple pipetting steps are not required. The system detects as few as 15 cells/well in a 384-well format in 10 minutes after adding reagent and mixing.

The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo® Assay generates a "glow-type" luminescent signal, which has a half-life generally greater than five hours, depending on cell type and medium used. The extended half-life eliminates the need to use reagent injectors and provides flexibility for continuous or batch mode processing of multiple plates. The unique homogeneous format avoids errors that may be introduced by other ATP measurement methods that require multiple steps.

The CellTiter-Glo® Luminescent Cell Viability Assay is a popular and trusted method used in a wide range of applications. 

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9155MA-mod
CellTiter-Glo® Luminescent Assay chemistry.
Line graph illustrating that the CellTiter-Glo® Assay offers 10-cell sensitivity (up to 5 logs linear range).

CellTiter-Glo® Assay offers 10-cell sensitivity; up to 5 logs linear range. The signal was read on a MyGlo™ Reagent Reader.

"We perform knockdown experiments for noncoding RNAs on a number of different cell lines to identify novel cancer therapy targets. The CellTiter-Glo® Assay is an extremely easy-to-use reagent to address cell viability and obtain consistent and reproducible results."

Anan Quan, Research Assistant, Beth Israel Deaconess Medical Center.

"The CellTiter-Glo® Assay provides a very robust, easy-to-use reagent for high-throughput screening of small molecules for antiviral activity against viruses such as SARS-CoV and SARS-CoV-2 that cause a cytopathic effect in cell lines."

Dr. Colleen Jonsson, Director of the Regional Biocontainment Laboratory and Director of the Institute for the Study of Host-Pathogen Systems, University of Tennessee Health Science Center.

Read more about Dr. Jonsson's work to identify antiviral agents here.


Notes

Using 100μl of CellTiter-Glo® Reagent per assay in a 96-well format, Cat.# G7570 is sufficient to perform 100 assays; Cat.# G7571 and G7572, 1,000 assays; Cat.# G7573, 10,000 assays. Using 25μl of CellTiter-Glo® Reagent per assay in a 384-well format, Cat.# G7570 is sufficient to perform 400 assays; Cat.# G7571 and G7572, 4,000 assays; Cat.# G7573, 40,000 assays.

Bioluminescence Resource Center

Learn more about how luminescence works, what makes it different from fluorescence and how you can use luminescence to fuel your own research.

Specifications

You are viewing: G7570 Change Configuration

What's in the box?

Item Part # Size

CellTiter-Glo® Substrate (lyophilized)

G755A 1 × 1 vial

CellTiter-Glo® Buffer

G756A 1 × 10ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

Specifications

You are viewing: G7571 Change Configuration

What's in the box?

Item Part # Size

CellTiter-Glo® Substrate (lyophilized)

G755A 10 × 1 vial

CellTiter-Glo® Buffer

G756A 10 × 10ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

Specifications

You are viewing: G7572 Change Configuration

What's in the box?

Item Part # Size

CellTiter-Glo® Substrate (lyophilized)

G755B 1 × 1 vial

CellTiter-Glo® Buffer

G756B 1 × 100ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

Specifications

You are viewing: G7573 Change Configuration

What's in the box?

Item Part # Size

CellTiter-Glo® Substrate (lyophilized)

G755B 10 × 1 vial

CellTiter-Glo® Buffer

G756B 10 × 100ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

Specifications

You are viewing: MG1010 Change Configuration

What's in the box?

Item Part # Size Available Separately

MyGlo™ Reagent Reader

  • USB Cable
  • Wall Power Adapter
  • Plug Adapters
MG1000 1 × 1 each

CellTiter-Glo® 2.0 Reagent

G9241 1 × 10ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.

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