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BacTiter-Glo™ Microbial Cell Viability Assay

A Luminescent Assay to Measure the Number of Viable Microbial Cells in Culture

  • A homogeneous, single reagent assay
  • Offers increased sensitivity, fast results

Size

Catalog number selected: G8230

$ 115.00 Your price: Log in

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BacTiter-Glo™ Microbial Cell Viability Assay
10ml
$ 115.00
Your price: Log in
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The BacTiter-Glo™ Microbial Cell Viability Assay provides a method for determining the number of viable microbial cells in culture based on quantitation of the ATP present. The homogeneous assay involves adding a single reagent (BacTiter-Glo™ Reagent) directly to bacterial cells cultured in medium and measuring luminescence. This assay format reduces pipetting errors that may be introduced during the multiple steps required of other ATP measurement methods.

The formulation of the reagent supports bacterial cell lysis and generation of a luminescent signal. The luminescent signal is proportional to the amount of ATP present, which is directly proportional to the number of viable cells in culture. The assay relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase) and a proprietary buffer formulation for extracting ATP from bacteria. The assay has been shown to detect a variety of bacteria and fungi.

Data can be recorded in 5 minutes or less after adding reagent and mixing. The assay's superior sensitivity allows the detection of growth or toxicity quickly after inoculation.

ATP can be measured from as few as 10 bacterial cells, and data is recorded by luminometer or CCD camera. The assay is 1,000-fold more sensitive than assays requiring absorbance readings. The "glow-type" luminescent signal is stable with a half-life generally greater than 30 minutes.

Applications of the assay include examining antimicrobial compound activity, screening antimicrobial compounds, examining bacterial growth with extended sensitivity and range, rapid bacterial quantitation, and rapid ATP detection.

 

Notes:
Using 100μl of BacTiter-Glo™ Reagent per assay in a 96-well format, Cat.# G8230 provides sufficient reagents to perform 100 assays; Cat.# G8231 and G8232, 1,000 assays; Cat.# G8233, 10,000 assays. Using 25μl of BacTiter-Glo™ Reagent per assay in a 384-well format, Cat.# G8230 provides sufficient reagents to perform 400 assays; Cat.# G8231 and G8232, 4,000 assays; Cat.# G8233, 40,000 assays. rATP (Cat.# P1132) must be purchased separately for performing a standard curve.

4609MA.eps
Overview of the BacTiter-Glo™ Microbial Cell Viability Assay protocol.

Evaluating bacterial growth using the BacTiter-Glo Assay.
Evaluating bacterial growth using the BacTiter-Glo™ Assay.

E. coli ATCC 25922 strain was grown in Mueller Hinton II (MH II) Broth at 37°C overnight. The overnight culture was diluted 1:106 in 50ml of fresh MH II Broth and incubated at 37°C with shaking at 250rpm. Samples were taken at various time points, and the BacTiter-Glo™ Assay was performed. Luminescence was recorded on a GloMax® 96 Microplate Luminometer. Optical density was measured at 600nm (O.D.600) using a Beckman DU650 spectrophotometer. Diluted samples were used when readings of RLU and O.D. exceeded 108 and 1, respectively.

Specifications

You are viewing: G8230 Change Configuration

What's in the box?

Item Part # Size

BacTiter-Glo™ Buffer

G818A 1 × 10ml

BacTiter-Glo™ Substrate

G819A 1 × 1 vial

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 7,422,868 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: G8231 Change Configuration

What's in the box?

Item Part # Size

BacTiter-Glo™ Buffer

G818A 10 × 10ml

BacTiter-Glo™ Substrate

G819A 10 × 1 vial

SDS

Choose language:

Certificate of Analysis

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View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 7,422,868 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: G8232 Change Configuration

What's in the box?

Item Part # Size

BacTiter-Glo™ Buffer

G818B 1 × 100ml

BacTiter-Glo™ Substrate

G819B 1 × 1 vial

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 7,422,868 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: G8233 Change Configuration

What's in the box?

Item Part # Size

BacTiter-Glo™ Buffer

G818B 10 × 100ml

BacTiter-Glo™ Substrate

G819B 10 × 1 vial

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 7,422,868 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

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