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Use of the BacTiterGlo Microbial Cell Viability Assay to Study Bacterial Attachment in Biofilm Formation

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Abstract

Biofilms are complex, sessile communities of bacteria that form on solid surfaces or at air-liquid interfaces. The formation of such biofilms follows a path of controlled steps, the first being attachment to the surface. To rigorously study the physiological changes that allow the bacteria to perform this first step, a quantitative assay that accurately determines the biomass of surface-attached bacteria is required. Here, we describe the use of the BacTiter-Glo™ Microbial Cell Viability Assay to measure biofilm attachment. This 96-well-format assay monitors intracellular ATP concentrations. Since bacterial cells maintain a relatively constant intracellular ATP concentration, this assay provides a reproducible relative measure of attached cells.

Promega Notes 99, 19–21.

Preeti Sule1, Tanush Wadhawan1, Alan J. Wolfe2, and Birgit M. Prüß1

1North Dakota State University, 2Loyola University Chicago

Publication Date: 2008

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