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GloMax® 20/20 Luminometer

Ultrasensitive Single Tube Luminometer

  • Two fluorescence modules available
  • Optional single or dual auto-injectors
  • Ideal for use with our bioluminescent cell health and gene reporter assays

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Catalog number selected: E5311

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GloMax® 20/20 Luminometer
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Ultra-Sensitive and Versatile Single Tube Luminometer

The GloMax® 20/20 Luminometer combines instrumentation and software in a complete solution that includes bioluminescent assays, protocols and support. In addition to high performance, the GloMax® 20/20 blends user-friendly operation and a small footprint with flexible purchasing options. The result of this design is an ultra-sensitive instrument with superior performance that is easy to use, affordable, and can be customized to your lab's needs.

  • Designed for use with any Promega bioluminescent assay
  • Touch screen interface provides comprehensive instrument control and data collection. 
  • Optional modules for fluorescence detection provide additional flexibility
  • Optional auto-injection system for added convenience meets the demands of the Dual-Luciferase® Assay



  • Plug-and-play usability
  • Preloaded protocols for easy implementation
  • Simple touch screen interface
  • Record data to printer or export to Excel® in real-time
  • Software wizard for setting up new protocols
  • Accessible injector system allows inspection of tubing and tips

  • Optional single or dual auto-injectors; measure flash or glow-type luminescence
  • Two fluorescent modules available
  • Accommodates 1.5ml microfuge tubes and 35mm petri dishes, with optional 12mm × 50mm test tube adapter
  • Measure light output from liquid and solid samples; no minimum volume requirements
Superior Sensitivity

  • Highest instrument sensitivity to measure the lowest assay signals
  • Low cross-talk between wells gives you better, more usable results
  • Broad dynamic range extends linear range of your assay; measure dim and bright samples without dilution

  • IQ/OQ Service available
  • Provides the required technical elements of a part 11 compliant system when used with the appropriate laboratory workflow:
    • User Authentication and Authorization
    • Data Integrity and Protection
    • Electronic Signatures
    • Audit Trails

Design Features

Instrument Specifications

To achieve the GloMax®-20/20's high sensitivity, the luminescence channel is positioned directly below the sample well. These conditions maximize light capture. In addition, a low-noise photomultiplier tube ensures that collected light is not compromised. The dynamic range of the GloMax®-20/20 is more than adequate to cover common luminescence applications, thus reducing the need to dilute samples.

The GloMax®-20/20 Luminometer Light Standard provides an external control to verify performance. Some labs require this additional verification procedure. Reading the light standard before taking measurements is a quick and easy way to ensure quality control of reproducibility, sensitivity, and linearity of measurements.

Detection Modes Luminescence, Fluorescence (optional)
Linear Dynamic Range >8 logs
Read Type Glow, Flash, Kinetic, Repeat
Sample Format 1.5ml microcentrifuge tubes, 35mm petri dishes, 12mm × 50mm test tubes (optional), minicell vials (optional, Fluorescence)
Sample Adapter Holds 1.5ml microcentrifuge tubes, optional 12mm × 50mm test tube holder available.
User Interface Touch screen navigation and operation
Data Output Data displayed on screen or connect to PC (not included) via serial cable to download. Optional thermal printer available.
External PC Requirements (optional) Compatible with 32- and 64-bit PCs using Windows® 7 Professional Service
Pack 1 with either Microsoft® Office 2010 Home and Business Edition (32- and 64-bit versions) or Microsoft® Office 2007 (32-bit version)
Computer Interface 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud rate
Power 12V DC 1.5A Max
Auto Shutoff Hibernates after 15 minutes of inactivity
Dimensions 12.92" D × 10.44" W × 8.42" H (32.82cm D × 26.52cm W × 21.39cm H)
Weight 8.4lbs (3.81kg)
Operating Temperature 60–105ºF (15–40ºC)
Warranty One year
Approvals CE
Single Auto Injector System Specifications
Number of Injectors One injector
Dispense Volume Range Selectable between 25–300µl in 5µl increments
Dual Auto Injector System Specifications
Number of Injectors Two injectors
Dispense Volume Range Selectable between 25–300µl in 5µl increments
Fluorescence Module Specifications

Optional Fluorescence Modules add functionality to the GloMax®-20/20. The modules are designed to measure common fluorescence applications using excitation wavelengths in the near UV or Blue light range. The Fluorescence Modules provide sensitive measurements of DNA, RNA, or protein quantitation dyes.

Light Sources Wavelength-matched LED
Detector PMT
UV Fluorescence Module
Wavelengths Ex 365nm, Em 440–470nm
Typical Fluorophores Hoechst dye, 4-methyl-umbelliferone (4-MU)
Detection Limit 10ng/ml dsDNA using Hoechst Dye 33258
Blue Fluorescence Module
Wavelengths Ex 460nm, Em 515–575nm
Typical Fluorophores EGFP, rAcGFP (DNA Quantitation, RNA Quantitation); Fluorescein (Protein Quantitation)
Detection Limit 450pg/ml dsDNA with DNA Quantitation Dye
Linear Dynamic Range 5 logs, assay dependent
Sample Adapter 100–200µl minicell vial
Read Out Fluorescence Standard Units, Direct Concentration
Calibration Single-point calibration
Discrete Sample Average Sample readings averaged over 5 seconds to improve accuracy

Frequently Asked Questions

GloMax® 20/20 Frequently Asked Questions
What is the price of the GloMax® 20/20 Luminometer?

The price may vary based on your application(s) and requirements. To receive a quote customized to your needs, use our online quotation form.

What is the detection system of the GloMax® 20/20 Luminometer?

The GloMax® 20/20 has a photomultiplier tube (PMT) detector. Because of its proprietary design, this detector offers both superior sensitivity and dynamic range.

How many injectors can be installed in the GloMax® 20/20 Luminometer?

The GloMax® 20/20 Luminometer accommodates one or two automatic injectors.

When/Why would I need an automatic injection system?

An automatic injection system is recommended when measuring flash luminescent reactions and when the assay requires high precision and reproducibility. Using an automatic injection system guarantees a consistent, precise injection rate and volume that is independent of the operator's skill or precision. Also, when working with a high sample load, automatic injection will save analysis time.

What are the advantages of internal automatic injectors versus external automatic injectors?

The GloMax® 20/20 internal automatic injectors permit dark-adaptation of the injectable reagents. The reagent supply remains in a protected, dark space inside the GloMax® 20/20 during the experiment. Because many reagents are light-sensitive, equilibrating the reagents to dark conditions allows for maximum reproducibility. Other luminometers rely on external automatic injectors, which draw reagents from a supply outside of the luminometer and do not protect reagents from the influence of ambient light. Internal injectors shorten the supply line length and reduce dead volume.

What types of test tubes can the GloMax® 20/20 Luminometer read?

The GloMax® 20/20 can read any clear 1.5ml microcentrifuge tubes, 35mm petri dishes, 12 × 50mm test tubes (optional) and minicell vials (optional, Fluorescence).

What operating software controls the GloMax® 20/20?

The GloMax® 20/20 has a touch screen-based Graphic User Interface (GUI). The GUI controls the instrument, reports data on the screen and sends data to a printer or computer.

What software comes with the GloMax® 20/20?

The GloMax® 20/20 includes a PC-based Spreadsheet Interface Software. This software transfers the data directly to an Excel® spreadsheet.

What operating systems are compatible with the GloMax® 20/20 Spreadsheet Interface Software?

The GloMax® 20/20 Spreadsheet Interface Software is compatible with Microsoft Windows® XP SP2 or higher.

Can I do kinetic measurements?

Yes, the current version of the GUI supports kinetic measurements.

Is there any other data handling functions that the GUI offers?

Yes, the GloMax® 20/20 GUI reports two RLU values and ratio of the firefly luciferase and Renilla luciferase signal for the Promega DLR™ Assay.

Is the software compatible with Macintosh® operating systems?

The Spreadsheet Interface Software is not compatible with Macintosh® Operating Systems.

For what applications can I use this instrument?

You can run all bioluminescent and chemiluminescent, flash and glow type of applications on the GloMax® 20/20.

The primary applications are: Reporter gene assays, ATP assays, Cytotoxicity, Cell proliferation, Nucleic acid quantitation, Enzyme assays, Intracellular Ca2+.

Optional Fluorescent Applications include: Nucleic Acid Quantitation and Protein Quantitation.

What is the sensitivity of the GloMax® 20/20 Luminometer?

The GloMax® 20/20 has a detection limit of 1 × 10–21 moles of firefly luciferase using the Promega Luciferase Assay Reagent.

How does the sensitivity of the GloMax® 20/20 compare to other luminometers?

The sensitivity of the GloMax® 20/20 is comparable or higher than the leading competitor's instrument. The GloMax® 20/20 is also 10 times higher in sensitivity to the TD-20/20 Single Tube Luminometer.

What is the dynamic range of the GloMax® 20/20?

The GloMax® 20/20 has over 8 logs of linear dynamic range.

How does the dynamic range of the GloMax® 20/20 compare to other instruments?

Other instruments on the market usually have a dynamic range of 6 logs.

What is the injection volume and precision of the GloMax® 20/20?

The injection volume is selectable between 25 and 300µl (±5µl); CV% >2%.

Do I need to change anything for power requirements in different countries?

No, the GloMax® 20/20 has an internal universal power supply. Simply use the proper AC adaptor.

What is the GloMax® 20/20 Light Standard?

The GloMax® 20/20 Light Standard is designed to provide a quick and easy way to verify the performance of the GloMax® 20/20. It allows you to check the reproducibility, sensitivity and linearity of a luminometer in less than 30 seconds. The Light Standard consists of three highly stable light sources that simulate luminescence samples at three different signal levels. It can be used for both short-term and long-term stability studies of the detection system.

What is the GloMax® 20/20 Fluorescent Module?

The GloMax® 20/20 Fluorescent Module is a snap-in device that allows you to measure fluorescent assays with GloMax® 20/20. There are two models, UV and BLUE.

Does the GloMax® 20/20 come with a Fluorescent Module or the Light Standard?

The Fluorescent Modules and the Light Standard are sold separately. You can purchase either the Fluorescent Module or the Light Standard when you order the instrument or anytime in the future.

Is it difficult to install a GloMax® 20/20 Fluorescent Module?

No, the GloMax® 20/20 Fluorescent Module is very easy to install and remove. It is a snap-in device and takes less than one minute to install.

What software do I need on my PC?

The GloMax® 20/20 Spreadsheet Interface Software is provided on a CD and is easy to install. You only need to have an available serial port to connect to the GloMax® 20/20. You must have MS Excel installed on your computer.

Does the instrument attach to a printer?

Yes, you can purchase an optional thermal printer and connect to the GloMax® 20/20. The data will be printed in real time.

Can I use my own printer with the GloMax® 20/20?

Yes, if the printer is a serial printer and can print maximum 80 characters. Promega also offers a thermal printer for purchase.

Does the GloMax® 20/20 Spreadsheet Interface Software work with older versions of Windows® software?

We tested the GloMax® 20/20 Spreadsheet Interface Software on Windows® XP SP2. The GloMax® 20/20 Spreadsheet Interface Software was successful in translating to MS Excel.


GloMax is a registered trademark of Promega Corporation. Macintosh is a registered trademark of Apple Computer, Inc. Excel and Windows are registered trademarks of Microsoft Corporation.

Available Separately

Product Catalog #
GloMax® 20/20 Light Standard E5341
GloMax® 20/20 Fluorescent Module, UV E5351
GloMax® 20/20 Fluorescent Module, Blue E5361
GloMax® 20/20 Test Tube Holder (1.5ml Microcentrifuge Tubes) E5371
GloMax® 20/20 Replacement Tubing (2), Valves (4), Tips (30) E4851*
GloMax® 20/20 Replacement Valves (4 sets) E5391
GloMax® 20/20 Replacement Power Supply E5411
Thermal Serial Printer and Universal Power Cable E2821
Thermal Printer Paper E2851
GloMax® 20/20 Sales Rental E5303
GloMax® 20/20 Base Instrument Service Agreement SA3000
GloMax® Injectors Service Agreement, 1 year SA3040

*Cat.# E4851 has replaced Cat.# E5381.


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