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Caspase-Glo® 3/7 Assay Systems

G8090
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Apoptosis Detection with Unmatched Speed and Sensitivity

  • High sensitivity—requires fewer cells and less enzyme
  • Simple add-mix-measure protocol—no sample preparation
  • Easily scalable to 96-, 384- and 1,536-well plate formats

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Catalog number selected: G8090

$ 135.00
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Caspase-Glo® 3/7 Assay Systems
2.5ml
$ 135.00
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A Homogeneous Assay That Measures Caspase-3/7 Activities

The Caspase-Glo® 3/7 assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimized for caspase-3/7 activity, luciferase activity and cell lysis. Adding the single Caspase-Glo® 3/7 Reagent in an "add-mix-measure" format results in cell lysis, followed by caspase cleavage of the substrate. This liberates free aminoluciferin, which is consumed by the luciferase, generating a "glow-type" luminescent signal that is proportional to caspase-3/7 activity.

The stabilized luciferase and proprietary buffer system improve assay performance across a wide range of assay conditions, and the assay is less likely to be affected by compound interference unlike fluorescent- or colorimetric-based assays. The Caspase-Glo® 3/7 Assay is designed for use with multiwell plate formats using either purified enzyme or cells in culture and can be multiplexed with other cell-based assays. The assay's high sensitivity allows for use in 3D cell culture systems. It also delivers excellent Z'-factor values in cell and purified enzyme models.

9555ma-01

Simple Bioluminescent Detection With A Plate-Reader

4059ma03-3b

Luminescence Is Linear Over a Broad Range of Cell Numbers


Multiplex with Cytotoxicity Assay in 3D Cell Cultures

HCT116 spheroids were created using the the InSphero GravityPLUSTM Hanging Drop System, and then treated with Panobinostat for up to 72 hours. CellToxTM Green was added at time of treatment. At 72 hours Caspase-Glo® 3/7 Reagent was added and plates were shaken for 30 minutes. Fluorescence was read at 485nm excitation, 520nm emission, followed by luminescence.

Notes

Using 100μl of Caspase-Glo® Reagent per assay in a 96-well format, Cat.# G8090 provides sufficient reagents to perform 25 assays; Cat.# G8091, 100 assays; Cat.# G8092 and G8093, 1,000 assays. Using 25μl of Caspase-Glo® Reagent per assay in a 384-well format, Cat.# G8090 provides sufficient reagents to perform 100 assays; Cat.# G8091, 400 assays; Cat.# G8092 and G8093, 4,000 assays.

Specifications

You are viewing: G8090 Change Configuration

What's in the box?

Item Part # Size

Caspase-Glo® 3/7 Buffer

G810C 1 × 2.5ml

Caspase-Glo® 3/7 Substrate

G811C 1 × 1 bottle

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

Specifications

You are viewing: G8091 Change Configuration

What's in the box?

Item Part # Size

Caspase-Glo® 3/7 Buffer

G810A 1 × 10ml

Caspase-Glo® 3/7 Substrate

G811A 1 × 1 bottle

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

Specifications

You are viewing: G8093 Change Configuration

What's in the box?

Item Part # Size

Caspase-Glo® 3/7 Buffer

G810A 10 × 10ml

Caspase-Glo® 3/7 Substrate

G811A 10 × 1 bottle

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

Specifications

You are viewing: G8092 Change Configuration

What's in the box?

Item Part # Size

Caspase-Glo® 3/7 Buffer

G810B 1 × 100ml

Caspase-Glo® 3/7 Substrate

G811B 1 × 1 bottle

SDS

Choose language:

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

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