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Factors to Consider for Designing and Optimizing Assays Applied to 3D Cultures

Dr. Terry Riss started the Cell Biology program at Promega Corporation in 1990 and held several R&D and Project Management positions since. Dr. Riss managed development of cell viability, cytotoxicity, apoptosis, and protease assay systems and also lead efforts to identify and promote multiplexing of cell-based assays to determine the mechanism of cell death.  Dr. Riss now serves as Global Strategic Marketing Manager, Cell Health involved in outreach educational training activities including validating assay systems applied to 3D cell culture models.
  • Terry Riss, PhD

  • Global Strategic Marketing Manager, Cell Health

  • Original Webinar Date: Tuesday, March 14, 2017

3D cell culture models present a challenge for using assay chemistries and imaging methods that were originally designed for measuring events from 2D monolayers of cells. In this webinar, we will present factors to consider when designing and optimizing assays applied to 3D cell culture models, case studies describing design of reagents to overcome the limitations inherent with detecting markers from large 3D culture models, and applications of novel real-time detection methods.

3D cell culture models are challenging when using assay chemistries and imaging methods originally designed for use with 2D cell culture models. The size of 3D structures, as well as the added complexity of scaffolds and matrix components used for culturing, often present barriers to directly measuring markers to assay biological function. In this webinar, we will present strategies and factors to consider when designing and validating assays applied to 3D cell culture models. We will also present case studies describing design of reagents and alternative approaches to overcome the limitations inherent with detecting markers from 3D culture models.

Topics we will cover include:

  • Designing detection reagents and appropriate controls to confirm lysis of all cells in 3D culture model systems
  • Measuring markers released into culture medium
  • The penetration of small molecule probes to interrogate biology within 3D structures
  • The potential to apply real-time multiplex detection methods with 3D models for high throughput screening