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Rapid Digestion–Trypsin and Rapid Digestion–Trypsin/Lys-C Kits

Minimize Protein Digestion Time, Maximize Results

  • Faster digestion time
  • Streamlined workflow
  • Tighter coefficients of variation

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Catalog number selected: VA1060

$ 208.00
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Rapid Digestion–Trypsin and Rapid Digestion–Trypsin/Lys-C Kits
Trypsin/1 × 100µg
$ 208.00
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Trypsin Digestion in as Little as 60 Minutes

The Rapid Digestion-Trypsin and Rapid Digestion-Trypsin/Lys-C Kits are designed to shorten protein digestion times to 60 minutes versus the typical 4–18 hours required for trypsin digestion. Both kits contain three components: i) protease (Trypsin or Trypsin/Lys-C Mix); ii) protease Resuspension Buffer; and iii) Rapid Digestion Buffer optimized for faster potein digestions. 

Trypsin digestion with these kits follows a simple-to-use protocol that is both fast and efficient. The protocol is flexible, can accommodate a large range of sample volumes and protein concentrations and requires no special laboratory equipment or off-line desalting. The entire sample preparation procedure is performed in as little as 60 minutes.

      Rapid Trypsin Digest overview
      Rapid Digestion Trypsin in J. Proteome Research

      Simple Process Enables Multiomic Analyses from a Common Cell Culture Sample

      The reach of omic analyses now includes the breadth of the cell life cycle, allowing study of processes from translation of RNA to protein, through proteome development and interactions, and metabolic activities. However, as Caprioli et al. (2018) note:

      "Many published methodologies for preparing samples for proteomic analysis by mass spectrometry are entirely incompatible with the analysis of metabolites or RNA from the same sample due to the presence of specific buffer components or detergents."

      The ideal, to study multiple omic systems from a single sample, is now possible using the process described in this Journal of Proteome Research paper: “An Integrated, High-Throughput Strategy for Multiomic Systems Level Analysis".

      In the paper Caprioli et al. used Rapid Digestion-Trypsin/Lys-C (described on this page) in their tryptic digestion of proteomic samples.

      Read the paper

      Trypsin Digestion in a Fraction of Standard Digestion Time

      Trypsin Digestion Standard Digestion Time

      Comparison of Peptide Spectral Matches: Rapid Digestion–Trypsin and Standard Digestion

      Rapid Digestion–Trypsin and Standard Digestion: Comparison of Peptide Spectral Matches

      The increase in spectra noted for Rapid Digestion–Trypsin sample preparation (30-minute digestion) indicates more complete digestion than standard overnight digestion (16 hours using Trypsin Gold, Mass Spectrometry Grade).

      Improved Sequence Coverage with Reduction and Alkylation Steps

      Improved Sequence Coverage with Reduction and Alkylation Steps

      Both Rapid Digestion–Trypsin and Rapid Digestion–Trypsin/Lys-C (35-minute digestions) are compatible with reduction and alkylation steps, resulting in increased sequence coverage.

      Specifications

      You are viewing: VA1060 Change Configuration

      What's in the box?

      Item Part # Size

      Resuspension Buffer

      V181A 1 × 500μl

      Rapid Trypsin Gold, MS Grade

      V528B 1 × 100μg

      Rapid Digest Buffer

      VA106A 1 × 30ml

      SDS

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      Use Restrictions

      For Research Use Only. Not for Use in Diagnostic Procedures.

      Storage Conditions

      BB

      Specifications

      You are viewing: VA1061 Change Configuration

      What's in the box?

      Item Part # Size

      Resuspension Buffer

      V181A 1 × 500μl

      Rapid Trypsin/Lys-C Mix, MS Grade

      V507C 1 × 100μg

      Rapid Digest Buffer

      VA106A 1 × 30ml

      SDS

      Choose language:

      Certificate of Analysis

      See all certificates for VA1061 Search
      View more results
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      Use Restrictions

      For Research Use Only. Not for Use in Diagnostic Procedures.

      Storage Conditions

      BB

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